Umbilical Cord Blood for Autologous Transfusion in the Early Postnatal Ontogeny: Analysis of Cell Composition and Viability during Long-Term Culturing

Verfasser / Beitragende:
[Yu. Romanov, E. Balashova, O. Bystrykh, K. Titkov, T. Dugina, N. Kabaeva, T. Fedorova, O. Rogachevskii, D. Degtyarev, G. Sukhikh]
Ort, Verlag, Jahr:
2015
Enthalten in:
Bulletin of Experimental Biology and Medicine, 158/4(2015-02-01), 523-527
Format:
Artikel (online)
ID: 605538387
LEADER caa a22 4500
001 605538387
003 CHVBK
005 20210128100901.0
007 cr unu---uuuuu
008 210128e20150201xx s 000 0 eng
024 7 0 |a 10.1007/s10517-015-2800-5  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s10517-015-2800-5 
245 0 0 |a Umbilical Cord Blood for Autologous Transfusion in the Early Postnatal Ontogeny: Analysis of Cell Composition and Viability during Long-Term Culturing  |h [Elektronische Daten]  |c [Yu. Romanov, E. Balashova, O. Bystrykh, K. Titkov, T. Dugina, N. Kabaeva, T. Fedorova, O. Rogachevskii, D. Degtyarev, G. Sukhikh] 
520 3 |a Changes in cell composition and viability as well as the content and functional activity of hemopoietic progenitor cells were analyzed during long-term (up to 1 month at 4°C) storage of human umbilical cord blood cells. No significant quantitative changes in erythrocytes were found during this period. The total content and viability of leukocytes changed, which resulted in the prevalence of mononuclear cells (lymphocytes and monocytes). Analysis of functional activity of hemopoietic stem cells in semisolid culture revealed a decrease in the relative content of CFU during the first week of culturing and inability of cells to colony formation after 2 weeks. 
540 |a Springer Science+Business Media New York, 2015 
690 7 |a umbilical cord blood  |2 nationallicence 
690 7 |a long-term culturing  |2 nationallicence 
690 7 |a leukocytes  |2 nationallicence 
690 7 |a viability  |2 nationallicence 
690 7 |a colony-forming units  |2 nationallicence 
700 1 |a Romanov  |D Yu  |u Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Balashova  |D E.  |u Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Bystrykh  |D O.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Titkov  |D K.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Dugina  |D T.  |u CryoCenter Umbilical Cord Blood Bank, Moscow, Russia  |4 aut 
700 1 |a Kabaeva  |D N.  |u Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Fedorova  |D T.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Rogachevskii  |D O.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Degtyarev  |D D.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Sukhikh  |D G.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
773 0 |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 158/4(2015-02-01), 523-527  |x 0007-4888  |q 158:4<523  |1 2015  |2 158  |o 10517 
856 4 0 |u https://doi.org/10.1007/s10517-015-2800-5  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s10517-015-2800-5  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Romanov  |D Yu  |u Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Balashova  |D E.  |u Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Bystrykh  |D O.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Titkov  |D K.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Dugina  |D T.  |u CryoCenter Umbilical Cord Blood Bank, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kabaeva  |D N.  |u Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Fedorova  |D T.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Rogachevskii  |D O.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Degtyarev  |D D.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Sukhikh  |D G.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 158/4(2015-02-01), 523-527  |x 0007-4888  |q 158:4<523  |1 2015  |2 158  |o 10517