caa a22 4500 605538387 CHVBK 20210128100901.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2800-5 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2800-5 Umbilical Cord Blood for Autologous Transfusion in the Early Postnatal Ontogeny: Analysis of Cell Composition and Viability during Long-Term Culturing [Elektronische Daten] [Yu. Romanov, E. Balashova, O. Bystrykh, K. Titkov, T. Dugina, N. Kabaeva, T. Fedorova, O. Rogachevskii, D. Degtyarev, G. Sukhikh] Changes in cell composition and viability as well as the content and functional activity of hemopoietic progenitor cells were analyzed during long-term (up to 1 month at 4°C) storage of human umbilical cord blood cells. No significant quantitative changes in erythrocytes were found during this period. The total content and viability of leukocytes changed, which resulted in the prevalence of mononuclear cells (lymphocytes and monocytes). Analysis of functional activity of hemopoietic stem cells in semisolid culture revealed a decrease in the relative content of CFU during the first week of culturing and inability of cells to colony formation after 2 weeks. Springer Science+Business Media New York, 2015 umbilical cord blood nationallicence long-term culturing nationallicence leukocytes nationallicence viability nationallicence colony-forming units nationallicence Romanov Yu Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut Balashova E. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut Bystrykh O. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Titkov K. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Dugina T. CryoCenter Umbilical Cord Blood Bank, Moscow, Russia aut Kabaeva N. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut Fedorova T. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Rogachevskii O. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Degtyarev D. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Sukhikh G. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 523-527 0007-4888 158:4<523 2015 158 10517 https://doi.org/10.1007/s10517-015-2800-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2800-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romanov Yu Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Balashova E. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Bystrykh O. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Titkov K. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Dugina T. CryoCenter Umbilical Cord Blood Bank, Moscow, Russia aut NATIONALLICENCE 700 1- Kabaeva N. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Fedorova T. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Rogachevskii O. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Degtyarev D. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Sukhikh G. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 523-527 0007-4888 158:4<523 2015 158 10517