caa a22 4500 605538417 CHVBK 20210128100902.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10517-015-2836-6 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2836-6 Effects of Acoustic and EHF Impulses on Multipotent Stromal Cells during Formation of Bone Marrow Containing Heterotopic Organs in Tissue Engineered Constructions [Elektronische Daten] [R. Chaikhalyan, V. Yusupov, Yu. Gorskaya, A. Kuralesova, Yu. Gerasimov, A. Sviridov, A. Tambiev, N. Vorob'eva, A. Shishkova, I. Moskvina, V. Bagratashvili] We studied the effects of physical factors (acoustic impulses of laser-induced hydrodynamics, AILIH, and EHF-radiation) on the formation of heterotopic bone marrow organs. Suspension of precipitated mouse bone marrow cells was exposed to AILIH and EHF or their combinations (AILIH+EHF, EHF+AILIH). The developed tissue engineering constructions (gelatin sponges containing 107 nucleated bone marrow cells exposed to physical factors) were transplanted under the renal capsule of syngeneic mice. Analysis of newly formed hemopoietic organs was performed after 3 and 5 months. The total amount of hemopoietic cells, number of multipotent stromal cells, efficiency of colony formation from these cells, and weight of bone capsule of the transplants were measured. Microscopic study showed that 5-month transplants were significantly larger than 3-month transplants and contained 3-fold more hemopoietic cells (20-fold in the AILIH+EHF group). The number of multipotent stromal cells was maximum in EHF+AILIH group (by 2.2 times higher than in the control) and minimum in AILIH+EHF group. Exposure to EHF+AILIH had most pronounced effect on the formation of the bone marrow transplants. The weight of bone capsules more rapidly increased in gelatin sponges of 3-month transplants of EHF+AILIH and AILIH groups. These data suggest that the studied physical factors can be used for acceleration of rehabilitation process. Springer Science+Business Media New York, 2015 organ containing bone marrow nationallicence multipotent stromal cells nationallicence heterotopic transplantation nationallicence EHF-radiation nationallicence acoustic impulses nationallicence Chaikhalyan R. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut Yusupov V. V. I. Il'ichev Pacific Oceanological Institute, Far-Eastern Division of Russian Academy of Sciences, Vladivostok, Russia aut Gorskaya Yu N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut Kuralesova A. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut Gerasimov Yu N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut Sviridov A. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia aut Tambiev A. Biological Faculty of M. V. Lomonosov Moscow State University, Moscow, Russia aut Vorob'eva N. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia aut Shishkova A. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut Moskvina I. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut Bagratashvili V. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/5(2015-03-01), 688-691 0007-4888 158:5<688 2015 158 10517 https://doi.org/10.1007/s10517-015-2836-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2836-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaikhalyan R. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Yusupov V. V. I. Il'ichev Pacific Oceanological Institute, Far-Eastern Division of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Gorskaya Yu N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Kuralesova A. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Gerasimov Yu N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Sviridov A. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Tambiev A. Biological Faculty of M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Vorob'eva N. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Shishkova A. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Moskvina I. N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Bagratashvili V. Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/5(2015-03-01), 688-691 0007-4888 158:5<688 2015 158 10517