Enhanced mechanical damage to in vitro cancer cells by high-intensity-focused ultrasound in the presence of microbubbles and titanium dioxide
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| 024 | 7 | 0 | |a 10.1007/s10396-015-0626-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10396-015-0626-4 | ||
| 245 | 0 | 0 | |a Enhanced mechanical damage to in vitro cancer cells by high-intensity-focused ultrasound in the presence of microbubbles and titanium dioxide |h [Elektronische Daten] |c [Katsuro Tachibana, Hitomi Endo, Loreto Feril, Seyedeh Nejad, Hiromasa Takahashi, Kyoichi Narihira, Toshihiro Kikuta] |
| 520 | 3 | |a Purpose: To evaluate in vitro the feasibility of therapeutic high-intensity-focused ultrasound (HIFU) combined with microbubbles and titanium dioxide (TiO2). Methods: Oral squamous cell carcinoma cells (HSC-2) were sonicated using a HIFU transducer with a resonant frequency of 3.5MHz, 30mm in diameter, and focal length of 50mm. The ultrasound intensity was 210W/cm2, and two pulses (0.5s each) were sonicated for each cell sample (9×104 cells per well). Immediately after HIFU, the viable cells were measured by an automated cell counter. The survival rate was measured in the presence of microbubbles (Sonazoid) and peroxo titania-silica (R-P-TS) or anatase titania-silica (R-A-TS) TiO2. Results: Cell viability immediately following sonication in the presence of TiO2 (R-A-TS) and TiO2 (R-P-TS) was 65.5±0.7 and 59.4±3.3%, respectively. A marked decrease in cell viability was seen when microbubbles were added to the above cell conditions. Specifically, cell viability decreased to 14.0±0.1 and 4.4±0.9% when microbubbles were added to samples containing TiO2 (R-A-TS) and TiO2 (R-P-TS), respectively. Conclusion: Immediate in vitro cell killing was observed with short pulsed duration HIFU sonication with a combination of microbubbles and TiO2. This finding suggests that TiO2 could have caused enhanced mechanical cell destruction by microbubbles. | |
| 540 | |a The Japan Society of Ultrasonics in Medicine, 2015 | ||
| 690 | 7 | |a High-intensity-focused ultrasound (HIFU) |2 nationallicence | |
| 690 | 7 | |a Titanium dioxide |2 nationallicence | |
| 690 | 7 | |a Microbubbles |2 nationallicence | |
| 690 | 7 | |a Cell damage |2 nationallicence | |
| 700 | 1 | |a Tachibana |D Katsuro |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Endo |D Hitomi |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Feril |D Loreto |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Nejad |D Seyedeh |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Takahashi |D Hiromasa |u Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Narihira |D Kyoichi |u Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Kikuta |D Toshihiro |u Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | |
| 773 | 0 | |t Journal of Medical Ultrasonics |d Springer Japan |g 42/4(2015-10-01), 449-455 |x 1346-4523 |q 42:4<449 |1 2015 |2 42 |o 10396 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10396-015-0626-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10396-015-0626-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tachibana |D Katsuro |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Endo |D Hitomi |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Feril |D Loreto |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nejad |D Seyedeh |u Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Takahashi |D Hiromasa |u Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Narihira |D Kyoichi |u Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kikuta |D Toshihiro |u Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Medical Ultrasonics |d Springer Japan |g 42/4(2015-10-01), 449-455 |x 1346-4523 |q 42:4<449 |1 2015 |2 42 |o 10396 | ||