caa a22 4500 605539812 CHVBK 20210128100908.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10396-015-0626-4 doi (NATIONALLICENCE)springer-10.1007/s10396-015-0626-4 Enhanced mechanical damage to in vitro cancer cells by high-intensity-focused ultrasound in the presence of microbubbles and titanium dioxide [Elektronische Daten] [Katsuro Tachibana, Hitomi Endo, Loreto Feril, Seyedeh Nejad, Hiromasa Takahashi, Kyoichi Narihira, Toshihiro Kikuta] Purpose: To evaluate in vitro the feasibility of therapeutic high-intensity-focused ultrasound (HIFU) combined with microbubbles and titanium dioxide (TiO2). Methods: Oral squamous cell carcinoma cells (HSC-2) were sonicated using a HIFU transducer with a resonant frequency of 3.5MHz, 30mm in diameter, and focal length of 50mm. The ultrasound intensity was 210W/cm2, and two pulses (0.5s each) were sonicated for each cell sample (9×104 cells per well). Immediately after HIFU, the viable cells were measured by an automated cell counter. The survival rate was measured in the presence of microbubbles (Sonazoid) and peroxo titania-silica (R-P-TS) or anatase titania-silica (R-A-TS) TiO2. Results: Cell viability immediately following sonication in the presence of TiO2 (R-A-TS) and TiO2 (R-P-TS) was 65.5±0.7 and 59.4±3.3%, respectively. A marked decrease in cell viability was seen when microbubbles were added to the above cell conditions. Specifically, cell viability decreased to 14.0±0.1 and 4.4±0.9% when microbubbles were added to samples containing TiO2 (R-A-TS) and TiO2 (R-P-TS), respectively. Conclusion: Immediate in vitro cell killing was observed with short pulsed duration HIFU sonication with a combination of microbubbles and TiO2. This finding suggests that TiO2 could have caused enhanced mechanical cell destruction by microbubbles. The Japan Society of Ultrasonics in Medicine, 2015 High-intensity-focused ultrasound (HIFU) nationallicence Titanium dioxide nationallicence Microbubbles nationallicence Cell damage nationallicence Tachibana Katsuro Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Endo Hitomi Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Feril Loreto Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Nejad Seyedeh Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Takahashi Hiromasa Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Narihira Kyoichi Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Kikuta Toshihiro Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut Journal of Medical Ultrasonics Springer Japan 42/4(2015-10-01), 449-455 1346-4523 42:4<449 2015 42 10396 https://doi.org/10.1007/s10396-015-0626-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10396-015-0626-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tachibana Katsuro Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Endo Hitomi Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Feril Loreto Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nejad Seyedeh Department of Anatomy, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Takahashi Hiromasa Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Narihira Kyoichi Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kikuta Toshihiro Department of Oral Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 773 0- Journal of Medical Ultrasonics Springer Japan 42/4(2015-10-01), 449-455 1346-4523 42:4<449 2015 42 10396