Ultrasound up-regulates expression of heme oxygenase-1 gene in endothelial cells
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605539820 | ||
| 003 | CHVBK | ||
| 005 | 20210128100908.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10396-015-0635-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10396-015-0635-3 | ||
| 245 | 0 | 0 | |a Ultrasound up-regulates expression of heme oxygenase-1 gene in endothelial cells |h [Elektronische Daten] |c [Ryohei Ogawa, Akihiko Watanabe, Akihiro Morii] |
| 520 | 3 | |a Purpose: We found heme oxygenase-1 (HO-1) gene up-regulation in response to ultrasound stimulation in endothelial cells. This project was started to explore the mechanism since it could be applied clinically. Methods: Quantitative real-time PCR experiments were conducted to determine the appropriate condition to up-regulate HO-1 gene expression. A promoter assay experiment and a microarray analysis were done to analyze signal transduction pathways to be activated in endothelial cells by ultrasound stimulation. Results: Approximately, 33-fold up-regulation of HO-1 mRNA was observed when endothelial cells were exposed to 1-MHz ultrasound at 0.3W/cm2 and DF 10% for 20s. Data obtained from promoter analysis and microarray analysis suggested that the Nrf2/StRE signal pathway was involved in the up-regulation. In addition, ultrasound stimulation to endothelial cells changed its gene expression profile so that it could modify the anti-inflammatory, antioxidant, and vasodilatory activities in addition to pro-angiogenic and vascular reconstitution activities of endothelial cells. Conclusion: It was suggested that ultrasound stimulation of endothelial cells up-regulated HO-1 expression through intracellular oxidative stress, which subsequently changed the gene expression pattern so that it could modify vascular functions. | |
| 540 | |a The Japan Society of Ultrasonics in Medicine, 2015 | ||
| 690 | 7 | |a Ultrasound |2 nationallicence | |
| 690 | 7 | |a Endothelial cells |2 nationallicence | |
| 690 | 7 | |a Heme oxygenase-1 |2 nationallicence | |
| 690 | 7 | |a Microarray |2 nationallicence | |
| 700 | 1 | |a Ogawa |D Ryohei |u Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, 930-0194, Toyama, Japan |4 aut | |
| 700 | 1 | |a Watanabe |D Akihiko |u Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan |4 aut | |
| 700 | 1 | |a Morii |D Akihiro |u Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan |4 aut | |
| 773 | 0 | |t Journal of Medical Ultrasonics |d Springer Japan |g 42/4(2015-10-01), 467-475 |x 1346-4523 |q 42:4<467 |1 2015 |2 42 |o 10396 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10396-015-0635-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10396-015-0635-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ogawa |D Ryohei |u Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, 930-0194, Toyama, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Watanabe |D Akihiko |u Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Morii |D Akihiro |u Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Medical Ultrasonics |d Springer Japan |g 42/4(2015-10-01), 467-475 |x 1346-4523 |q 42:4<467 |1 2015 |2 42 |o 10396 | ||