miR-26a enhances autophagy to protect against ethanol-induced acute liver injury
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| 024 | 7 | 0 | |a 10.1007/s00109-015-1282-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00109-015-1282-2 | ||
| 245 | 0 | 0 | |a miR-26a enhances autophagy to protect against ethanol-induced acute liver injury |h [Elektronische Daten] |c [Weidong Han, Xianghui Fu, Jiansheng Xie, Zhipeng Meng, Ying Gu, Xichun Wang, Ling Li, Hongming Pan, Wendong Huang] |
| 520 | 3 | |a Autophagy is a process for the turnover of intracellular organelles and molecules during stress responses. microRNAs (miRNAs) are small, non-coding endogenous RNAs that may regulate almost every cellular process. However, the roles of miRNAs in autophagy are still poorly understood. In this study, we show that miR-26a enhances autophagy in both culture cells and the mouse liver. Hepatic overexpression of miR-26a in mice alleviated ethanol-induced hepatic steatosis and liver injury. Overexpression of miR-26a increased the expression of the autophagy mediator Beclin-1, which is regulated by mitogen-activated protein kinases (MAPKs). We identified DUSP4 and DUSP5, two MAPKs inhibitors, as direct targets of miR-26a. We further demonstrated that miR-26a targeted the 3′-UTRs of several other negative regulators of autophagy. Our results thus identify a novel miRNA-mediated mechanism that enhances cytoprotective autophagy in the liver. Key messages: • miR-26a enhances autophagy in liver cells. • Hepatic overexpression of miR-26a in mice alleviates ethanol-induced liver injury. • Overexpression of miR-26a increases the expression of autophagy mediator Beclin-1. • DUSP4 and DUSP5, two MAPKs inhibitors, were identified as direct targets of miR-26a. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a miR-26a |2 nationallicence | |
| 690 | 7 | |a Autophagy |2 nationallicence | |
| 690 | 7 | |a Hepatic steatosis |2 nationallicence | |
| 690 | 7 | |a Mitogen-activated protein kinases |2 nationallicence | |
| 690 | 7 | |a Ethanol binge |2 nationallicence | |
| 690 | 7 | |a 3′-UTR : 3′ untranslated region |2 nationallicence | |
| 690 | 7 | |a miRNAs : MicroRNAs |2 nationallicence | |
| 690 | 7 | |a miR-26a : MicroRNA 26a |2 nationallicence | |
| 690 | 7 | |a MAPKs : Mitogen-activated protein kinases |2 nationallicence | |
| 690 | 7 | |a LDs : Lipid droplets |2 nationallicence | |
| 690 | 7 | |a mRNAs : Messenger RNAs |2 nationallicence | |
| 690 | 7 | |a CQ : Chloroquine |2 nationallicence | |
| 690 | 7 | |a tf-LC3 : Tandem fluorescent-tagged LC3 |2 nationallicence | |
| 690 | 7 | |a PCR : Real-time polymerase chain reaction |2 nationallicence | |
| 690 | 7 | |a ALT : Alanine aminotransferase |2 nationallicence | |
| 690 | 7 | |a TEM : Transmission electron microscopy |2 nationallicence | |
| 700 | 1 | |a Han |D Weidong |u Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 East Qingchun Road, 310016, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Fu |D Xianghui |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | |
| 700 | 1 | |a Xie |D Jiansheng |u Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 East Qingchun Road, 310016, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Meng |D Zhipeng |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | |
| 700 | 1 | |a Gu |D Ying |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | |
| 700 | 1 | |a Wang |D Xichun |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | |
| 700 | 1 | |a Li |D Ling |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | |
| 700 | 1 | |a Pan |D Hongming |u Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 East Qingchun Road, 310016, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Huang |D Wendong |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | |
| 773 | 0 | |t Journal of Molecular Medicine |d Springer Berlin Heidelberg |g 93/9(2015-09-01), 1045-1055 |x 0946-2716 |q 93:9<1045 |1 2015 |2 93 |o 109 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00109-015-1282-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00109-015-1282-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Han |D Weidong |u Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 East Qingchun Road, 310016, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fu |D Xianghui |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xie |D Jiansheng |u Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 East Qingchun Road, 310016, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Meng |D Zhipeng |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gu |D Ying |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Xichun |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Ling |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pan |D Hongming |u Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 East Qingchun Road, 310016, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Wendong |u Division of Molecular Diabetes Research, Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute, City of Hope National Medical Center, 1500 E. Duarte Road, 91010, Duarte, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Molecular Medicine |d Springer Berlin Heidelberg |g 93/9(2015-09-01), 1045-1055 |x 0946-2716 |q 93:9<1045 |1 2015 |2 93 |o 109 | ||