Quantitative Spin-trapping ESR Investigation of Alkoxyl Radical Derived from AAPH: Development of a Flow-injection Spin-trapping ESR System for the Oxygen Radical Absorbance Capacity Assay
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| 024 | 7 | 0 | |a 10.1007/s00723-015-0664-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00723-015-0664-5 | ||
| 245 | 0 | 0 | |a Quantitative Spin-trapping ESR Investigation of Alkoxyl Radical Derived from AAPH: Development of a Flow-injection Spin-trapping ESR System for the Oxygen Radical Absorbance Capacity Assay |h [Elektronische Daten] |c [Akira Nakajima, Tomoko Yamaguchi, Tomoyuki Yamashita, Kiyoshi Kawai, Yusuke Miyake, Kenji Kanaori, Kunihiko Tajima] |
| 520 | 3 | |a A flow-injection electron spin resonance (ESR) system was developed for the quantitative detection of an alkoxyl radical (RO·, R=C(CH3)2-C(+NH2Cl−)NH2) derived from AAPH (2,2′-azobis(2,4-amidinopropane) dihydrochloride) by thermal decomposition. Optimal measurement conditions for the system were examined, and it was found that the control of the radical formation by quenching in an ice-water vessel should be effective to obtain the stable and accurate results. The system was applied for the estimation of the alkoxyl radical-elimination ability and the oxygen radical absorbance capacity values of selected biosubstances, such as Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), caffeic acid, 4-hydroxycinnamic acid, epinephrine, rutin, (+)-catechin, l-tryptophane, and d-mannitol. As the rate constant for the reaction between alkoxyl radical and substrate, k S, could not be unequivocally determined, the defined value, γ 50=k S/k 1, (k 1, the rate constant for the reaction between alkoxyl radical and spin trap) was used as a reaction parameter of substrate for alkoxyl radical. The flow-injection electron spin resonance system using alkoxyl radical elimination gave valid γ 50 values for biosubstances, and the system should be a valuable method for the evaluation of the antioxidant ability. | |
| 540 | |a Springer-Verlag Wien, 2015 | ||
| 700 | 1 | |a Nakajima |D Akira |u Department of Biotechnology, Frontier Science Research Center, University of Miyazaki, Kiyotake, 889-1692, Miyazaki, Japan |4 aut | |
| 700 | 1 | |a Yamaguchi |D Tomoko |u Venture Laboratory, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Yamashita |D Tomoyuki |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Kawai |D Kiyoshi |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Miyake |D Yusuke |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Kanaori |D Kenji |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Tajima |D Kunihiko |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | |
| 773 | 0 | |t Applied Magnetic Resonance |d Springer Vienna |g 46/9(2015-09-01), 1013-1022 |x 0937-9347 |q 46:9<1013 |1 2015 |2 46 |o 723 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00723-015-0664-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00723-015-0664-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nakajima |D Akira |u Department of Biotechnology, Frontier Science Research Center, University of Miyazaki, Kiyotake, 889-1692, Miyazaki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yamaguchi |D Tomoko |u Venture Laboratory, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yamashita |D Tomoyuki |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawai |D Kiyoshi |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Miyake |D Yusuke |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kanaori |D Kenji |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tajima |D Kunihiko |u Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Magnetic Resonance |d Springer Vienna |g 46/9(2015-09-01), 1013-1022 |x 0937-9347 |q 46:9<1013 |1 2015 |2 46 |o 723 | ||