Probing Radical Chemistry in Salmonella typhimurium Cells Under Oxidative Stress Using Spin Traps and Nitroxyl Radicals

Verfasser / Beitragende:
[Marco Bellinazzi, Stephen Batchelor, Georg Gescheidt]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Magnetic Resonance, 46/3(2015-03-01), 239-249
Format:
Artikel (online)
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024 7 0 |a 10.1007/s00723-014-0631-6  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00723-014-0631-6 
245 0 0 |a Probing Radical Chemistry in Salmonella typhimurium Cells Under Oxidative Stress Using Spin Traps and Nitroxyl Radicals  |h [Elektronische Daten]  |c [Marco Bellinazzi, Stephen Batchelor, Georg Gescheidt] 
520 3 |a The interaction between tert-butyl hydroperoxide and Salmonella typhimurium cell strain TA1547 as monitored by electron spin resonance (ESR) is reported. Spin traps (DMPO and POBN) show the formation of methyl and alkoxyl radicals in the bacterial culture. The kinetic behavior of the radical formation was followed by the dose-dependent decay of the ESR spectra of the stable nitroxide radicals TEMPOL and 3-aminomethyl-PROXYL, which are quenched by short-lived radicals. Whereas the spin traps provide qualitative characterization of some of the radicals produced by the interaction between the bacteria and the hydroperoxide, our investigation indicates that the use of nitroxides provides further quantitative kinetic data. 
540 |a Springer-Verlag Wien, 2015 
700 1 |a Bellinazzi  |D Marco  |u Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria  |4 aut 
700 1 |a Batchelor  |D Stephen  |u Unilever Research, Port Sunlight, Quarry Road East, Bebington, CH63 3JW, Wirral, UK  |4 aut 
700 1 |a Gescheidt  |D Georg  |u Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria  |4 aut 
773 0 |t Applied Magnetic Resonance  |d Springer Vienna  |g 46/3(2015-03-01), 239-249  |x 0937-9347  |q 46:3<239  |1 2015  |2 46  |o 723 
856 4 0 |u https://doi.org/10.1007/s00723-014-0631-6  |q text/html  |z Onlinezugriff via DOI 
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900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00723-014-0631-6  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Bellinazzi  |D Marco  |u Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Batchelor  |D Stephen  |u Unilever Research, Port Sunlight, Quarry Road East, Bebington, CH63 3JW, Wirral, UK  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Gescheidt  |D Georg  |u Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Magnetic Resonance  |d Springer Vienna  |g 46/3(2015-03-01), 239-249  |x 0937-9347  |q 46:3<239  |1 2015  |2 46  |o 723