caa a22 4500 60554574X CHVBK 20210128100937.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00723-014-0631-6 doi (NATIONALLICENCE)springer-10.1007/s00723-014-0631-6 Probing Radical Chemistry in Salmonella typhimurium Cells Under Oxidative Stress Using Spin Traps and Nitroxyl Radicals [Elektronische Daten] [Marco Bellinazzi, Stephen Batchelor, Georg Gescheidt] The interaction between tert-butyl hydroperoxide and Salmonella typhimurium cell strain TA1547 as monitored by electron spin resonance (ESR) is reported. Spin traps (DMPO and POBN) show the formation of methyl and alkoxyl radicals in the bacterial culture. The kinetic behavior of the radical formation was followed by the dose-dependent decay of the ESR spectra of the stable nitroxide radicals TEMPOL and 3-aminomethyl-PROXYL, which are quenched by short-lived radicals. Whereas the spin traps provide qualitative characterization of some of the radicals produced by the interaction between the bacteria and the hydroperoxide, our investigation indicates that the use of nitroxides provides further quantitative kinetic data. Springer-Verlag Wien, 2015 Bellinazzi Marco Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria aut Batchelor Stephen Unilever Research, Port Sunlight, Quarry Road East, Bebington, CH63 3JW, Wirral, UK aut Gescheidt Georg Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria aut Applied Magnetic Resonance Springer Vienna 46/3(2015-03-01), 239-249 0937-9347 46:3<239 2015 46 723 https://doi.org/10.1007/s00723-014-0631-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00723-014-0631-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bellinazzi Marco Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria aut NATIONALLICENCE 700 1- Batchelor Stephen Unilever Research, Port Sunlight, Quarry Road East, Bebington, CH63 3JW, Wirral, UK aut NATIONALLICENCE 700 1- Gescheidt Georg Institute of Physical and Theoretical Chemistry, NAWI Graz, Graz University of Technology, Technikerstrasse 4/I, 8010, Graz, Austria aut NATIONALLICENCE 773 0- Applied Magnetic Resonance Springer Vienna 46/3(2015-03-01), 239-249 0937-9347 46:3<239 2015 46 723