caa a22 4500 605545812 CHVBK 20210128100937.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00723-015-0710-3 doi (NATIONALLICENCE)springer-10.1007/s00723-015-0710-3 Intracerebral Antioxidant Ability of Neonatal Rats After Acute Hypoxic-Ischemic Brain Injury Estimated Using the Brain Homogenate-ESR Method [Elektronische Daten] [Akira Nakajima, Li Yang, Emiko Matsuda, Ayako Nagano, Hiroshi Sameshima, Tsuyomu Ikenoue] The intracerebral antioxidant ability of neonatal rats after acute hypoxic-ischemic (HI) brain injury was estimated using the brain homogenate-ESR method after intraperitoneal injection of 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl, blood-brain-barrier permeable stable cyclic nitroxide. Seven-day-old Wistar rats were subjected to a modified Levine's procedure for producing HI brain injury. The rats 7days after HI insult were ranked in severe, mild and normal groups based on their brain damage. The severe brain damage was observed with high probability. The intracerebral antioxidant ability of the normal and mild groups was same as that of the control rats. On the other hand, the antioxidant ability in the ligated side of the cerebral hemisphere of the severe group was significantly lower than that of the control rats. The HI insult resulted in the oxidative stress and the insufficient supply of the endogenous antioxidants, which caused the significant decrease in the intracerebral antioxidant ability. The brain homogenate-ESR method can be easily conducted for a large number of neonatal rats at regular time intervals, which indicates the usefulness of the method to evaluate the intracerebral antioxidant ability of neonatal rats. Springer-Verlag Wien, 2015 Nakajima Akira Department of Biotechnology, Frontier Science Research Center, University of Miyazaki, 889-1692, Kiyotake, Miyazaki, Japan aut Yang Li Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut Matsuda Emiko Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut Nagano Ayako Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut Sameshima Hiroshi Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut Ikenoue Tsuyomu Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut Applied Magnetic Resonance Springer Vienna 46/10(2015-10-01), 1079-1088 0937-9347 46:10<1079 2015 46 723 https://doi.org/10.1007/s00723-015-0710-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00723-015-0710-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakajima Akira Department of Biotechnology, Frontier Science Research Center, University of Miyazaki, 889-1692, Kiyotake, Miyazaki, Japan aut NATIONALLICENCE 700 1- Yang Li Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut NATIONALLICENCE 700 1- Matsuda Emiko Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut NATIONALLICENCE 700 1- Nagano Ayako Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut NATIONALLICENCE 700 1- Sameshima Hiroshi Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut NATIONALLICENCE 700 1- Ikenoue Tsuyomu Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, 889-1692, Kiyotake, Miyazaki, Japan aut NATIONALLICENCE 773 0- Applied Magnetic Resonance Springer Vienna 46/10(2015-10-01), 1079-1088 0937-9347 46:10<1079 2015 46 723