caa a22 4500 606154345 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9448-7 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9448-7 In vivo construction of tissue-engineered cartilage using adipose-derived stem cells and bioreactor technology [Elektronische Daten] [Hongjun Kang, Shibi Lu, Jiang Peng, Qiang Yang, Shuyun Liu, Li Zhang, Jingxiang Huang, Xiang Sui, Bin Zhao, Aiyuan Wang, Wenjing Xu, Quanyi Guo, Qing Song] The present study aims to investigate the feasibility of tissue-engineered cartilage constructed in vivo and in vitro by dynamically culturing adipose-derived stem cells (ADSCs) with an articular cartilage acellular matrix in a bioreactor and subsequently implanting the cartilage in nude mice. ADSCs were proliferated, combined with three dimensional scaffolds (cell density: 5×107/mL) and subsequently placed in a bioreactor and culture plate for 3weeks. In the in vivo study, complexes cultured for 1week under dynamic or static states were subcutaneously implanted into nude mice and collected after 3weeks. Indicators such as gross morphology, histochemistry and immunohistochemistry were examined. In the in vitro study, histological observation showed that most scaffolds in the dynamic group were absorbed, and cell proliferation and matrix secretion were significant. Positive staining of safranin-O and alcian blue II collagen stain in the dynamic group was significantly stronger than that in the static culture group. In the in vivo study, cartilage-like tissues formed in the specimens of the two groups. Histological examination showed that cell distribution in the dynamic group was relatively more uniform than in the static group, and matrix secretion was relatively stronger. Bioreactor culturing can promote ADSC proliferation and cartilage differentiation and is thus a suitable method for constructing tissue-engineered cartilage in vivo. Springer Science+Business Media Dordrecht, 2014 Adipose-derived stem cells nationallicence Bioreactors nationallicence Bracket nationallicence Cartilage nationallicence In vivo nationallicence Nude mice nationallicence ADSCs : Adipose-derived stem cells nationallicence PBS : Phosphate-buffered saline nationallicence DMEM : Dulbecco's modified Eagle's medium nationallicence FBS : Foetal bovine serum nationallicence IACUC : Institutional Animal Care and Use Committee nationallicence bFGF : Basic fibroblast growth factor nationallicence Kang Hongjun Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Lu Shibi Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Peng Jiang Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Yang Qiang Department of Spine Surgery, Tianjin Hospital, 300211, Tianjin, China aut Liu Shuyun Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Zhang Li Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Huang Jingxiang Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Sui Xiang Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Zhao Bin Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Wang Aiyuan Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Xu Wenjing Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Guo Quanyi Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Song Qing Critical Care Medicine, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 123-133 1389-9333 16:1<123 2015 16 10561 https://doi.org/10.1007/s10561-014-9448-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9448-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang Hongjun Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Lu Shibi Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Peng Jiang Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Yang Qiang Department of Spine Surgery, Tianjin Hospital, 300211, Tianjin, China aut NATIONALLICENCE 700 1- Liu Shuyun Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Zhang Li Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Huang Jingxiang Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Sui Xiang Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Zhao Bin Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Wang Aiyuan Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Xu Wenjing Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Guo Quanyi Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 700 1- Song Qing Critical Care Medicine, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, 100853, Beijing, China aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 123-133 1389-9333 16:1<123 2015 16 10561