caa a22 4500 606154361 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9456-7 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9456-7 Incidence of bacterial contamination and predisposing factors during bone and tendon allograft procurement [Elektronische Daten] [Clara Terzaghi, Alessia Longo, Claudio Legnani, Davide Bernasconi, Maristella Faré] The aim of this study was to analyze factors contributing to bacteriological contamination of bone and tendon allograft. Between 2008 and 2011, 2,778 bone and tendon allografts obtained from 196 organ and tissue donors or tissue donors only were retrospectively analysed. Several variables were taken into account: donor type (organ and tissue donors vs. tissue donor), cause of death, time interval between death and tissue procurement, duration of the procurement procedure, type of allografts, number of team members, number of trainees members, associated surgical procedures, positivity to haemoculture, type of procurement. The overall incidence of graft contamination was 23%. The cause of death, the procurement time, the duration of procurement, the associated surgical procedures were not associated with increased risk of contamination. Significant effect on contamination incidence was observed for the number of staff members performing the procurement. In addition, our study substantiated significantly higher contamination rate among bone allografts than from tendon grafts. According to these observations, in order to minimize the contamination rate of procured musculoskeletal allografts, we recommend appropriate donor selection, use of standard sterile techniques, immediate packaging of each allograft to reduce graft exposure. Allograft procurement should be performed by a small surgical team. Springer Science+Business Media Dordrecht, 2014 Allograft nationallicence Bacterial contamination nationallicence Procurement nationallicence Tissue banking nationallicence Terzaghi Clara Musculoskeletal Tissue Bank of Milan, G. Pini Orthopaedic Institute, Milan, Italy aut Longo Alessia Musculoskeletal Tissue Bank of Milan, G. Pini Orthopaedic Institute, Milan, Italy aut Legnani Claudio Minimally Invasive Articular Surgery Unit, G. Pini Orthopaedic Institute, Milan, Italy aut Bernasconi Davide Department of Health Sciences, University of Milan-Bicocca, Monza, Italy aut Faré Maristella Musculoskeletal Tissue Bank of Milan, G. Pini Orthopaedic Institute, Milan, Italy aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 151-157 1389-9333 16:1<151 2015 16 10561 https://doi.org/10.1007/s10561-014-9456-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9456-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Terzaghi Clara Musculoskeletal Tissue Bank of Milan, G. Pini Orthopaedic Institute, Milan, Italy aut NATIONALLICENCE 700 1- Longo Alessia Musculoskeletal Tissue Bank of Milan, G. Pini Orthopaedic Institute, Milan, Italy aut NATIONALLICENCE 700 1- Legnani Claudio Minimally Invasive Articular Surgery Unit, G. Pini Orthopaedic Institute, Milan, Italy aut NATIONALLICENCE 700 1- Bernasconi Davide Department of Health Sciences, University of Milan-Bicocca, Monza, Italy aut NATIONALLICENCE 700 1- Faré Maristella Musculoskeletal Tissue Bank of Milan, G. Pini Orthopaedic Institute, Milan, Italy aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 151-157 1389-9333 16:1<151 2015 16 10561