caa a22 4500 60615437X CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9457-6 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9457-6 Immortalised human mesenchymal stem cells undergo chondrogenic differentiation in alginate and PGA/PLLA scaffolds [Elektronische Daten] [K. Stölzel, G. Schulze-Tanzil, H. Olze, S. Schwarz, E. Feldmann, N. Rotter] Adult mesenchymal stem cells (MSCs) are a promising cell source in tissue engineering due to their availability, ease of isolation and high proliferative activity. This study was undertaken to investigate whether immortalised human MSC are able to undergo chondrogenic differentiation when cultured in alginate or in resorbable scaffolds. We directly compared chondrogenesis MSCs with that of human nasoseptal chondrocytes. Two previously established human stem cell lines L87/4 and V54-2 immortalised using the SV40 large T-antigen were either cultured in alginate or in polyglycolic acid/poly-l-lactic acid (PGA/PLLA) (90/10) copolymer scaffolds. TGF-β1 was added for induction of chondrogenesis. Human nasoseptal chondrocytes and human fibroblasts were used as controls. Cultures were analysed for sulfated glycosaminoglycans (alcian blue staining) and for the presence of collagen type I, II and X (immunolabelling). SV40 large T-antigen immortalised human MSCs have the potential to undergo chondrogenic differentiation: After 21days, cartilage-specific type II collagen was present in alginate and PGA/PLLA scaffolds, independent of the addition of TGF-β1. Collagen type X was present in monolayer cultures as well as in alginate and PGA/PLLA scaffolds. Collagen type I was produced in marginal amounts only. Immortalised human MSCs are a suitable tool to study chondrogenesis in vitro and to screen biomaterials for cartilage tissue engineering applications. Springer Science+Business Media Dordrecht, 2014 Tissue engineering nationallicence Mesenchymal stem cells nationallicence Chondrogenesis nationallicence Nasoseptal chondrocytes nationallicence Alginate nationallicence Polyglycolic acid/poly- l -lactic acid (PGA/PLLA) nationallicence Stölzel K. Department of Otorhinolaryngology, Head and Neck Surgery, Charité-University of Medicine, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany aut Schulze-Tanzil G. Department for Orthopaedic, Trauma and Reconstructive Surgery, Charité-University of Medicine, Campus Benjamin Franklin, Garystraße 5, 14195, Berlin, Germany aut Olze H. Department of Otorhinolaryngology, Head and Neck Surgery, Charité-University of Medicine, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany aut Schwarz S. Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89070, Ulm, Germany aut Feldmann E. Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89070, Ulm, Germany aut Rotter N. Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89070, Ulm, Germany aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 159-170 1389-9333 16:1<159 2015 16 10561 https://doi.org/10.1007/s10561-014-9457-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9457-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stölzel K. Department of Otorhinolaryngology, Head and Neck Surgery, Charité-University of Medicine, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Schulze-Tanzil G. Department for Orthopaedic, Trauma and Reconstructive Surgery, Charité-University of Medicine, Campus Benjamin Franklin, Garystraße 5, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Olze H. Department of Otorhinolaryngology, Head and Neck Surgery, Charité-University of Medicine, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Schwarz S. Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89070, Ulm, Germany aut NATIONALLICENCE 700 1- Feldmann E. Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89070, Ulm, Germany aut NATIONALLICENCE 700 1- Rotter N. Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89070, Ulm, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 159-170 1389-9333 16:1<159 2015 16 10561