caa a22 4500 606154388 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9447-8 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9447-8 The effect of supercritical carbon dioxide sterilization on the anisotropy of bovine cortical bone [Elektronische Daten] [Nicholas Russell, Alain Rives, Matthew Pelletier, Tian Wang, William Walsh] Bone allografts are used to replace bone that has been removed or to augment bone tissue in a number of clinical scenarios. In order to minimize the risk of infection and immune response, the bone is delipidated and terminally sterilized prior to implantation. The optimal method for bone graft sterilization has been the topic of considerable research and debate. Recently, supercritical carbon dioxide (SCCO2) treatments have been shown to terminally sterilize bone against a range of bacteria and viruses. This study aimed to evaluate the effect of these SCCO2 treatments on the anisotropic mechanical properties of cortical bone. Adult bovine cortical cubes were prepared and treated using SCCO2 and a range of common processing additives (ethanol, peracetic acid and hydrogen peroxide). The bone was mechanically tested in uniaxial compression in the axial, radial and tangential orientations. Ultimate stress, strain, elastic modulus, energy and stiffness were evaluated. This study found that SCCO2 treatment without additive did not alter the ultimate stress, stiffness or energy to failure depreciably in any orientation. The addition of sterilants peracetic acid and hydrogen peroxide also preserved mechanical function, with no deleterious effect on stress or stiffness. This study highlights the expediency of SCCO2 treatment for bone allograft processing as terminal sterilization can be achieved while maintaining the intrinsic mechanical properties of the graft. Springer Science+Business Media Dordrecht, 2014 Sterilization nationallicence Allograft nationallicence Bone nationallicence Mechanical nationallicence Anisotropy nationallicence Supercritical fluid nationallicence Russell Nicholas Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut Rives Alain Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut Pelletier Matthew Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut Wang Tian Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut Walsh William Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 109-121 1389-9333 16:1<109 2015 16 10561 https://doi.org/10.1007/s10561-014-9447-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9447-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Russell Nicholas Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut NATIONALLICENCE 700 1- Rives Alain Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut NATIONALLICENCE 700 1- Pelletier Matthew Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut NATIONALLICENCE 700 1- Wang Tian Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut NATIONALLICENCE 700 1- Walsh William Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 109-121 1389-9333 16:1<109 2015 16 10561