caa a22 4500 606154396 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9438-9 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9438-9 Effects of different doses of gamma irradiation on oxygen and water vapour transmission rate of preserved human amniotic membrane [Elektronische Daten] [Nor Zahari, Suzina Sheikh Ab Hamid, Norimah Yusof] Preserved human amniotic membrane either air dried or glycerol preserved has been used effectively to treat superficial and partial thickness wounds without leaving any obvious hypertrophic scar. The preserved amnion, sterilised by ionising radiation, is known as an effective barrier for heat, fluid and protein loss while adheres nicely on wound. Air drying slightly reduced the oxygen transmission rate (OTR) of the amnion and the value significantly dropped after 15kGy (p<0.05). Glycerol preservation significantly reduced (p<0.05) the OTR indicating less oxygen transmitted through the well structured cells of the amnion. Increase in the OTR with the increasing radiation doses up to 35kGy possibly due to direct effects of radiation that resulted in large intercellular gaps. Both preservation methods significantly increased (p<0.05) the water vapour transmission rate (WVTR). However, the low WVTR in the air dried amnion at 15 and 25kGy was postulated due to cross-linking of collagen. Changes in the biophysical properties can be linked to direct and indirect effects of radiation on collagen bundles. The radiation dose of 25kGy caused no adverse effect on biophysical properties hence it is still acceptable to sterilize both the air dried and the glycerol preserved amnions. Springer Science+Business Media Dordrecht, 2014 Human amniotic membrane nationallicence Air dried nationallicence Glycerol preserved nationallicence Gamma radiation nationallicence SEM nationallicence Water vapour transmission rate nationallicence Oxygen transmission rate nationallicence Cell morphology nationallicence Zahari Nor Tissue Bank, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia aut Sheikh Ab Hamid Suzina Tissue Bank, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia aut Yusof Norimah Malaysian Nuclear Agency, Bangi, 43000, Kajang, Selangor, Malaysia aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 55-63 1389-9333 16:1<55 2015 16 10561 https://doi.org/10.1007/s10561-014-9438-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9438-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zahari Nor Tissue Bank, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia aut NATIONALLICENCE 700 1- Sheikh Ab Hamid Suzina Tissue Bank, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia aut NATIONALLICENCE 700 1- Yusof Norimah Malaysian Nuclear Agency, Bangi, 43000, Kajang, Selangor, Malaysia aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 55-63 1389-9333 16:1<55 2015 16 10561