caa a22 4500 606154426 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9442-0 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9442-0 Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone [Elektronische Daten] [Christian Fölsch, Wolfram Mittelmeier, Thomas von Garrel, Uwe Bilderbeek, Nina Timmesfeld, Axel Pruss, Hans-Peter Matter] Thermodisinfection of human femoral heads from living donors harvested during hip joint replacement is an established processing procedure. This study was designed to examine the influence of heat sterilization on pull out strength of cancellous bone and storage at different temperatures up to 2years since we had previously studied the storage of unprocessed cancellous bone. Porcine cancellous bone resembling human bone structure was obtained from 140 proximal humerus of 6-8months old piglets. Pull out strength of screws after thermodisinfection was compared with unprocessed cancellous bone and tested immediately and after 6, 12 and 24months of storage at −20 and −80°C. A three-way ANOVA was performed and significance level was 5%. The thermodisinfected bone showed a pull out force of 2729N (1657-3568N). The reduction of pull out strength compared with unprocessed bone over all periods of storage was 276N on average with 95% confidence interval ranging from 166N to 389N (p<0.0001). Different freezing temperatures did not influence this mechanic property within 24months storage and showed no difference compared with fresh frozen bone. Thermodisinfection of cancellous bone preserves tensile strength necessary for clinical purposes. The storage at −20°C for at least 2years did not show relevant decrease of pull out strength compared with −80°C without difference between thermodisinfected and fresh frozen bone. The increase of the storage temperature to −20°C for at least 2years should be considered. Springer Science+Business Media Dordrecht, 2014 Thermodisinfection nationallicence Allograft bone nationallicence Storage temperature nationallicence Duration of storage nationallicence Pull out strength cancellous bone nationallicence Fölsch Christian Department of Orthopaedic Surgery, Philipps University Medical School, Philipps University Marburg, Baldingerstrasse, 35043, Marburg, Germany aut Mittelmeier Wolfram Department of Orthopaedic Surgery, University Medical School, Doberaner Strasse, 18057, Rostock, Germany aut von Garrel Thomas Department of Orthopaedic and Trauma Surgery, St. Willehad Hospital, Ansgaristrasse 12, 26382, Wilhelmshaven, Germany aut Bilderbeek Uwe Bahnhofstrasse 2, 35321, Laubach, Germany aut Timmesfeld Nina Institute of Medical Biometry and Epidemiology, Philipps University Marburg, Bunsenstrasse 3, 35037, Marburg, Germany aut Pruss Axel University Tissue Bank, Institute of Transfusion Medicine, Charité University Medical School, Charitéplatz 1, 10117, Berlin, Germany aut Matter Hans-Peter GNG - Gesundheitsnetz Gießen, Frankfurter Strasse 1, 35390, Gießen, Germany aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 73-81 1389-9333 16:1<73 2015 16 10561 https://doi.org/10.1007/s10561-014-9442-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9442-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fölsch Christian Department of Orthopaedic Surgery, Philipps University Medical School, Philipps University Marburg, Baldingerstrasse, 35043, Marburg, Germany aut NATIONALLICENCE 700 1- Mittelmeier Wolfram Department of Orthopaedic Surgery, University Medical School, Doberaner Strasse, 18057, Rostock, Germany aut NATIONALLICENCE 700 1- von Garrel Thomas Department of Orthopaedic and Trauma Surgery, St. Willehad Hospital, Ansgaristrasse 12, 26382, Wilhelmshaven, Germany aut NATIONALLICENCE 700 1- Bilderbeek Uwe Bahnhofstrasse 2, 35321, Laubach, Germany aut NATIONALLICENCE 700 1- Timmesfeld Nina Institute of Medical Biometry and Epidemiology, Philipps University Marburg, Bunsenstrasse 3, 35037, Marburg, Germany aut NATIONALLICENCE 700 1- Pruss Axel University Tissue Bank, Institute of Transfusion Medicine, Charité University Medical School, Charitéplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Matter Hans-Peter GNG - Gesundheitsnetz Gießen, Frankfurter Strasse 1, 35390, Gießen, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 73-81 1389-9333 16:1<73 2015 16 10561