caa a22 4500 606154434 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9444-y doi (NATIONALLICENCE)springer-10.1007/s10561-014-9444-y Adding value to rare tissue samples donated to biobanks: characterisation of breast tissue and primary cell cultures obtained from a female-to-male transgender patient [Elektronische Daten] [Rebecca Millican-Slater, Rona Good, Claire Nash, Judith Heads, Steven Pollock, Rebecca Chalkley, Jenny Gomm, J. Jones, Sreekumar Sundara-Rajan, Kieran Horgan, Andrew Hanby, Valerie Speirs] Biobanks provide a window of opportunity to store and add value to material from rare cases allowing their future use in biomedical research. One such example is the opportunityto obtain good quality tissue from patients undergoing gender re-assignment. Following patient agreement to donate tissue samples to our biobank we catalogued the histological appearance, defined the expression of the hormone receptors ERα, PR, AR and the proliferation marker Ki67, and generated and characterised primary cell cultures in a female to male (FTM) transgender patient referred to our unit for surgery. Immunohistochemistry was performed for ERα, PR and AR and the proliferation marker Ki67. Hormone receptor expression was confined to epithelial cells lining the breast ducts. Ki67 immunoreactivity was sparse indicating little proliferation of luminal epithelium, consistent with normal mammary gland. Cultures of epithelial cells and fibroblasts were derived from surplus tissue. The latter lacked expression of epithelial markers and hormone receptors but exhibited expression of vimentin. Culture of the former on Matrigel saw an outgrowth of more rounded "epithelial-like” cells. Immunofluoresence characterisation showed a mixed phenotype with expression of vimentin and both myoepithelial and luminal epithelial markers. Sporadic weak ERα expression and moderate PR expression was seen. In summary, as well as routinely collecting tissue and blood samples, we have characterised and stored tissue and cells from a FTM transgender patient, adding value to this resource which,available from the Breast Cancer Campaign Tissue Bank for those interested in further studying the biology of FTM transgender tissue. Springer Science+Business Media Dordrecht, 2014 Breast tissue nationallicence Transgender nationallicence Cell culture nationallicence Tissue bank nationallicence Biobank nationallicence Rare nationallicence Millican-Slater Rebecca Department of Histopathology and Molecular Pathology, St James's Institute of Oncology, St James's University Hospital, Leeds, UK aut Good Rona Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Nash Claire Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Heads Judith Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Pollock Steven Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Chalkley Rebecca Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Gomm Jenny Barts Cancer Institute, London, UK aut Jones J. Barts Cancer Institute, London, UK aut Sundara-Rajan Sreekumar Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Horgan Kieran Leeds Breast Unit, St James's University Hospital, Leeds, UK aut Hanby Andrew Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Speirs Valerie Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 27-34 1389-9333 16:1<27 2015 16 10561 https://doi.org/10.1007/s10561-014-9444-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9444-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Millican-Slater Rebecca Department of Histopathology and Molecular Pathology, St James's Institute of Oncology, St James's University Hospital, Leeds, UK aut NATIONALLICENCE 700 1- Good Rona Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Nash Claire Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Heads Judith Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Pollock Steven Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Chalkley Rebecca Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Gomm Jenny Barts Cancer Institute, London, UK aut NATIONALLICENCE 700 1- Jones J. Barts Cancer Institute, London, UK aut NATIONALLICENCE 700 1- Sundara-Rajan Sreekumar Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Horgan Kieran Leeds Breast Unit, St James's University Hospital, Leeds, UK aut NATIONALLICENCE 700 1- Hanby Andrew Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Speirs Valerie Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 27-34 1389-9333 16:1<27 2015 16 10561