caa a22 4500 606154442 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9451-z doi (NATIONALLICENCE)springer-10.1007/s10561-014-9451-z Effects of continuous passage on immunomodulatory properties of human adipose-derived stem cells [Elektronische Daten] [Xiyou Wang, Cuilong Liu, Shaodan Li, Yong Xu, Ping Chen, Yi Liu, Qiang Ding, Wasilijiang Wahapu, Baofa Hong, Minghui Yang] Human adipose-derived stem cells (hADSCs) have the ability to influence immune response, and hence are key cell sources for tissue repair and regeneration. In this study we explored the effect of continuous passage on the immunomodulatory properties of hADSCs to provide some advises for large-scale production of hADSCs for clinical applications. We found that after continuous passage, the specific surface markers expression levels as well as the adipogenic and osteogenic differentiation capacities of hADSCs had no obvious changes. However, the secretion levels of IL-10 and HGF reduced dramatically along with passage numbers. Furthermore, the INF-γ level was found higher in which medium peripheral blood mononuclear cells were co-cultured with hADSCs with higher passage numbers. Also, the in vivo experiments showed that the peritonitis model mice, which were injected with higher passage numbers of hADSCs, tended to have higher levels of inflammation. All these together indicated that continuous passage has only minor effect on the cell phenotypes but will impair the immunomodulatory properties of hADSCs. This suggests that hADSCs could be prepared by continuous passage, but only those cells of lower passage numbers would be ideal therapeutic tools. Springer Science+Business Media Dordrecht, 2014 Human adipose-derived stem cells nationallicence Continuous passage nationallicence Phenotype nationallicence Immunomodulatory property nationallicence Wang Xiyou Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Liu Cuilong Department of Urology, PLA Navy General Hospital, No 6 Fucheng Road, Hai Dian District, 100048, Beijing, People's Republic of China aut Li Shaodan Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Xu Yong Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Chen Ping Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Liu Yi Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Ding Qiang Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Wahapu Wasilijiang Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Hong Baofa Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Yang Minghui Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 143-150 1389-9333 16:1<143 2015 16 10561 https://doi.org/10.1007/s10561-014-9451-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9451-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Xiyou Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Cuilong Department of Urology, PLA Navy General Hospital, No 6 Fucheng Road, Hai Dian District, 100048, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Shaodan Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xu Yong Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Ping Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yi Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Ding Qiang Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wahapu Wasilijiang Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Hong Baofa Department of Urology, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yang Minghui Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, No 28 Fuxing Road, Hai Dian District, 100853, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 143-150 1389-9333 16:1<143 2015 16 10561