caa a22 4500 606154485 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9446-9 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9446-9 Radioprotective effect of N -acetyl-L-cysteine free radical scavenger on compressive mechanical properties of the gamma sterilized cortical bone of bovine femur [Elektronische Daten] [Farzaneh Allaveisi, Bijan Hashemi, Seyed Mortazavi] Gamma sterilization of bone allografts is used as a gold standard method to provide safety against disease transmission. However, it is well documented that high dose levels of ionizing radiation can degrade bone mechanical properties. This effect, which is attributed to the formation of free radicals through radiolysis of the water content of collagen, can lead to post-implantation difficulties such as pre-failure and/or secondary fractures of bone allografts. Recently, treatment of irradiated allografts with free radical scavengers is used to protect them against radiation-induced damages. This study aimed to investigate the radioprotective role of N-acetyl-L-cysteine (NAC) during the gamma sterilization of the cortical bone of bovine femurs using the compressive test. Totally, 195 cubic specimens with a dimension of 5×5×3cubicmm were divided into 13 groups including a control and 12 experimental groups exposed to 18, 36, and 70kGy at three different NAC concentrations (1.25, 12.5, and 25mM for 18kGy; 5, 50, and 100mM for 36kGy; 10, 100, and 200mM for 70kGy). The mechanical behavior of the sterilized specimens was studied using the uniaxial compressive test. The results indicated a concentration-dependent radioprotection effect of NAC on the plastic properties of the cortical bones. The concentration dependency of NAC was in turn related to radiation dose levels. In conclusion, treatment of bone specimens with a characteristic concentration of NAC during exposure to specific radiation dose levels can provide an efficient radioprotection window for preserving the mechanical stability of gamma sterilized allografts. Springer Science+Business Media Dordrecht, 2014 Gamma sterilization nationallicence Cortical bone nationallicence Free radical scavenger nationallicence Compression test nationallicence Allograft nationallicence Mechanical properties nationallicence Allaveisi Farzaneh Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Al-Ahmad and Chamran Cross, P.O. Box 14115-111, 1411713116, Tehran, Iran aut Hashemi Bijan Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Al-Ahmad and Chamran Cross, P.O. Box 14115-111, 1411713116, Tehran, Iran aut Mortazavi Seyed Joint Reconstruction Research Center, Tehran University of Medical Sciences, Tehran, Iran aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 97-108 1389-9333 16:1<97 2015 16 10561 https://doi.org/10.1007/s10561-014-9446-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9446-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Allaveisi Farzaneh Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Al-Ahmad and Chamran Cross, P.O. Box 14115-111, 1411713116, Tehran, Iran aut NATIONALLICENCE 700 1- Hashemi Bijan Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Al-Ahmad and Chamran Cross, P.O. Box 14115-111, 1411713116, Tehran, Iran aut NATIONALLICENCE 700 1- Mortazavi Seyed Joint Reconstruction Research Center, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 97-108 1389-9333 16:1<97 2015 16 10561