caa a22 4500 606154493 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9443-z doi (NATIONALLICENCE)springer-10.1007/s10561-014-9443-z Assessment of an improved bone washing protocol for deceased donor human bone [Elektronische Daten] [M. Eagle, J. Man, P. Rooney, P. Hogg, J. Kearney] NHSBT Tissue Services issues bone to surgeons in the UK in two formats, fresh-frozen unprocessed bone from living donors and processed bone from deceased donors. Processed bone may be frozen or freeze dried and all processed bone is currently subjected to a washing protocol to remove blood and bone marrow. In this study we have improved the current bone washing protocol for cancellous bone and assessed the success of the protocol by measuring the removal of the bone marrow components: soluble protein, DNA and haemoglobin at each step in the process, and residual components in the bone at the end of the process. The bone washing protocol is a combination of sonication, warm water washes, centrifugation and chemical (ethanol and hydrogen peroxide) treatments. We report that the bone washing protocol is capable of removing up to 99.85% soluble protein, 99.95% DNA and 100% of haemoglobin from bone. The new bone washing protocol does not render any bone cytotoxic as shown by contact cytotoxicity assays. No microbiological cell growth was detected in any of the wash steps. This process is now in use for processed cancellous bone issued by NHSBT. Springer Science+Business Media Dordrecht, 2014 Cancellous bone nationallicence Washing protocol nationallicence Soluble protein nationallicence DNA nationallicence Haemoglobin nationallicence Allograft nationallicence Eagle M. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Man J. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Rooney P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Hogg P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Kearney J. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 83-90 1389-9333 16:1<83 2015 16 10561 https://doi.org/10.1007/s10561-014-9443-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9443-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eagle M. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 700 1- Man J. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 700 1- Rooney P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 700 1- Hogg P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 700 1- Kearney J. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 83-90 1389-9333 16:1<83 2015 16 10561