caa a22 4500 606154507 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10561-014-9449-6 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9449-6 Inactivation of glycogen synthase kinase-3β up-regulates β-catenin and promotes chondrogenesis [Elektronische Daten] [Junjie Zhou, Yan Chen, Chengfu Cao, Xianqi Chen, Wenwu Gao, Lei Zhang] This study aimed to investigate whether inhibition of glycogen synthase kinase-3β (GSK-3β) could promote chondrocytes proliferation. The expression pattern of GSK-3β was firstly determined by immunohistochemistry (IHC) in normal mouse. Tibias were then isolated and cultured for 6days. The tibias were treated with dimethylsulfoxide (control) or GSK-3 inhibitor SB415286 (SB86). Length of tibias was measured until 6days after treatment. These bones were either stained with alcian blue/alizarin red or analyzed by IHC. In addition, GSK-3β and β-catenin were analyzed by Western blot. Finally, cartilage-specific GSK-3β deletion mice (KO) were generated. Efficiency of GSK-3β deletion was determined through Western blot and IHC. After treated by inhibitor SB86, the overall length of growth plate was not changed. However, growth of tibia in SB86 group was increased by 31%, the length of resting and proliferating was increased 13% (P<0.01), whereas the length of hypertrophic was decreased by 57% (P<0.01). Besides, the mineralized length was found to be significant longer than the control group (P<0.05). In KO mice, growth plate and calvaria tissue both exhibit significant reduction of GSK-3β (P<0.05) whereas the lengths of tibias in KO were almost same compared with control mice. Finally, an increase amount of β-catenin protein was observed in SB86 (P<0.05). In addition, significantly increased β-catenin was also found in the growth plate of KO mice (P<0.05). Inhibition of GSK-3 could promote longitudinal growth of bone through increasing bone formation. Besides, the inactivation of GSK-3β could lead to enhancing β-catenin, therefore promote chondrocytes proliferation. Springer Science+Business Media Dordrecht, 2014 Glycogen synthase kinase-3β nationallicence β-catenin nationallicence Tibia nationallicence Chondrogenesis nationallicence Zhou Junjie Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut Chen Yan Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut Cao Chengfu Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut Chen Xianqi Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut Gao Wenwu Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut Zhang Lei Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 135-142 1389-9333 16:1<135 2015 16 10561 https://doi.org/10.1007/s10561-014-9449-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9449-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Junjie Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Chen Yan Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Cao Chengfu Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Chen Xianqi Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Gao Wenwu Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Lei Orthopedic Surgery, PuTuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164, LanXi Road, 200062, Shanghai, China aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/1(2015-03-01), 135-142 1389-9333 16:1<135 2015 16 10561