caa a22 4500 606154515 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9479-0 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9479-0 Development of a terminally sterilised decellularised dermis [Elektronische Daten] [P. Hogg, P. Rooney, S. Leow-Dyke, C. Brown, E. Ingham, J. Kearney] Many of the decellularised dermis products on the market at present are aspectically produced. NHS Blood and Transplant Tissue Services have developed a method of producing a dCELL human dermis which has been terminally sterilised by gamma irradiation. The terminally sterilised decellularised dermis was compared with cellular tissue and examined for histology, residual DNA content, biomechanical and biochemical properties, in vitro cytotoxicity and in vivo implantation in a mouse model. No alterations in morphology as viewed by light microscopy were observed and DNA removal was 99%. There were no significant changes in ultimate tensile stress or evidence for collagen denaturation or cytotoxicity. The in vivo studies did not indicate any adverse tissue reactions in the mouse model and demonstrated incorporation of dCELL human dermis into the host. Decellularisation, followed by terminal sterilisation with gamma irradiation, is an appropriate method to produce a human dermis allograft material suitable for transplantation. Springer Science+Business Media Dordrecht, 2014 Dermis nationallicence Human nationallicence Tissue nationallicence Decellularised nationallicence Irradiation nationallicence Sterilisation nationallicence Hogg P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Rooney P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Leow-Dyke S. IBMR, University of Leeds, Leeds, UK aut Brown C. IBMR, University of Leeds, Leeds, UK aut Ingham E. IBMR, University of Leeds, Leeds, UK aut Kearney J. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 351-359 1389-9333 16:3<351 2015 16 10561 https://doi.org/10.1007/s10561-014-9479-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9479-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hogg P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 700 1- Rooney P. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 700 1- Leow-Dyke S. IBMR, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Brown C. IBMR, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Ingham E. IBMR, University of Leeds, Leeds, UK aut NATIONALLICENCE 700 1- Kearney J. NHSBT, Tissue Services R&D, 14 Estuary Banks, L24 8RB, Speke, Liverpool, UK aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 351-359 1389-9333 16:3<351 2015 16 10561