caa a22 4500 60615454X CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9484-3 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9484-3 Dehydration of corneal anterior donor tissue with polyethylene glycol (PEG)-enriched media [Elektronische Daten] [Jessica Lie, Claire Monnereau, Esther Groeneveld-van Beek, Jacqueline van der Wees, Johannes Frank, Marieke Bruinsma, Gerrit Melles] Anterior donor grafts (including scleral rim, without Descemet membrane) increase in thickness and become hazy upon storage in organ culture (OC) medium. Transfer of these grafts to standard dehydration media just before transplantation does not reduce their thickness to normal. Therefore, we assessed the efficacy of different media enriched with polyethylene glycol (PEG) as dehydrating agents for organ-cultured anterior donor grafts. Grafts were harvested and stored in the commercial OC medium ‘Max' (without dextran) for 1week, and subsequently dehydrated in the standard commercial dehydration medium ‘Jet' (with dextran) supplemented with 4-20% PEG3350, or ‘Max' supplemented with 20% PEG6000 and PEG20.000, or 5-20% PEG35.000. Central corneal thickness (CCT), as assessed by anterior segment-optical coherence tomography, and transparency were evaluated before, and at 1, 4 and 7days of dehydration. Transfer of grafts after 1week of OC (average 1,200µm) to ‘Jet' supplemented with PEG3350 revealed a concentration-dependent effect of dehydration; CCT was restored to normal (500-600µm) when 10% PEG3350 was added. However, transparency was only temporarily restored; after 1day, the grafts turned hazy. In contrast, grafts transferred to ‘Max' supplemented with 20% PEG35.000 were transparent throughout the evaluation period, but were dehydrated to beyond normal levels (average 300µm). ‘Max' supplemented with 5% PEG35.000 dehydrated grafts to normal values and restored transparency throughout. Thus, dehydration of anterior donor grafts prior to surgery in dextran-free OC medium supplemented with 5% PEG35.000 reduces graft thickness to normal and may facilitate anterior keratoplasty procedures. Springer Science+Business Media Dordrecht, 2014 Cornea, anterior donor graft nationallicence Dehydration nationallicence Eye bank nationallicence Polyethylene glycol nationallicence Lie Jessica Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut Monnereau Claire Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands aut Groeneveld-van Beek Esther Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut van der Wees Jacqueline Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut Frank Johannes Delft University of Technology, Delft, The Netherlands aut Bruinsma Marieke Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut Melles Gerrit Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 399-409 1389-9333 16:3<399 2015 16 10561 https://doi.org/10.1007/s10561-014-9484-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9484-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lie Jessica Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Monnereau Claire Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Groeneveld-van Beek Esther Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- van der Wees Jacqueline Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Frank Johannes Delft University of Technology, Delft, The Netherlands aut NATIONALLICENCE 700 1- Bruinsma Marieke Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Melles Gerrit Amnitrans EyeBank Rotterdam, Rotterdam, The Netherlands aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 399-409 1389-9333 16:3<399 2015 16 10561