caa a22 4500 606154558 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9476-3 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9476-3 Neurogenic differentiation from adipose-derived stem cells and application for autologous transplantation in spinal cord injury [Elektronische Daten] [Yong Zhao, Hui Jiang, Xin-wei Liu, Jian-Ting Chen, Liang-Bi Xiang, Da-Peng Zhou] Mesenchymal stem cells derived from adipose tissue have the capacity to differentiate into endodermal, mesoderm and ectodermal cell lineages in vitro, which are an ideal engraft in tissue-engineered repair. In this study, mouse adipose-derived stem cells (ADSCs) were isolated from subcutaneous fat. The markers of ADSCs, CD13, CD29, CD44, CD71, CD73, CD90, CD105, CD166, Nestin, GFAP and MAP-2 were detected by immunofluorescence assays. The ADSCs were cultured in cocktail factors (including ATRA, GGF-2, bFGF, PDGF and forskolin) for neurogenic differentiation. The neurogenic cells markers, Nestin, GFAP and MAP-2 were analyzed using immunofluorescence and real-time PCR after dramatic changes in morphology. Neurogenic cells from ADSCs were autologous transplanted into the mouse of spinal cord injury for observation neurogenic cells colonization in spinal cord. The result demonstrated that the mouse ADSCs were positive for the CD13, CD29, CD44, CD71, CD73, CD90, CD105 and CD166 but negative for neurogenic cell markers, MAP-2, GFAP and Nestin. After neurogenic differentiation, the neurogenic cells were positive for neurogenic cell special markers, gene expression level showed a time-lapse increase, and the cells were successful colonized into spinal cord. In conclusion, our research shows that a population of neuronal cells can be specifically generated from ADSCs and that induced cells may allow for participation in tissue-repair. Springer Science+Business Media Dordrecht, 2014 Adipose-derived stem cells nationallicence Neurogenic differentiation nationallicence Autologous transplantation nationallicence Zhao Yong Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut Jiang Hui Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, 110016, Shenyang, Liaoning Province, China aut Liu Xin-wei Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut Chen Jian-Ting Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, 110016, Shenyang, Liaoning Province, China aut Xiang Liang-Bi Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut Zhou Da-Peng Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 335-342 1389-9333 16:3<335 2015 16 10561 https://doi.org/10.1007/s10561-014-9476-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9476-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Yong Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Jiang Hui Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, 110016, Shenyang, Liaoning Province, China aut NATIONALLICENCE 700 1- Liu Xin-wei Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Chen Jian-Ting Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, 110016, Shenyang, Liaoning Province, China aut NATIONALLICENCE 700 1- Xiang Liang-Bi Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 700 1- Zhou Da-Peng Department of Orthopedics, Nanfang Hospital of Southern Medical University, 510515, Guangzhou, Guangdong Province, China aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 335-342 1389-9333 16:3<335 2015 16 10561