caa a22 4500 606154574 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9477-2 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9477-2 Evaluation of a new protocol for sterility controls of corneal culture medium [Elektronische Daten] [H. Thomasen, F. Mosel, K.-P. Steuhl, D. Meller] Careful testing for microbial contamination is essential for corneal transplants. Sterility tests are performed on the antibiotics containing culture medium leaving the problem that antibiotics might compromise the test results. In this study a protocol for the application of the automated BacT/Alert system for sterility testing of corneal cell culture medium was examined. Corneal culture medium in combination with an antibiotics degrading enzyme were injected in resin containing test bottles of the BacT/Alert system named FA plus (intended for aerobic microorganisms) or FN plus (intended for anaerobic microorganisms) depending on their aerobic or anaerobic nature. Additionally i-FA plus(aerobic test bottle for industrial use) bottles were used. Microbial test strains on the basis of the European Pharmacopaea (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, Aspergillus brasiliensis and Clostridium sporogenes) with the addition of Propioniobacterium acnes were added to the test bottles. The bottles were incubated at two different temperatures for 14days. The time to detection (TTD) was monitored for each bottle. Growth of the test strains except European Pharm was detected in the FA and FN Plus bottles. The TTD for the strains was 44±1.5h (P. aeruginosa), 57.7±2.2h (B. subtilis), 56±1h (S. aureus), 26.3±1h (C. sporogenes), 223±4.6h (P. acnes), 64.4±10 (C. albicans). A. brasiliensis was detected in i-FA Plus bottles with a TTD of 94.9±3.7h. The application of BacT/Alert FA Plus and FN Plus resin bottles in combination with a penicillin degrading enzyme is able to detect small scale microbial contamination with different microorganisms in antibiotic containing corneal culture medium. For detection of Aspergillus brasiliensis in the medium the (i-) FA Plus bottles should be used. Springer Science+Business Media Dordrecht, 2014 Cornea nationallicence Eye banking nationallicence Culture media nationallicence Sterility test nationallicence Thomasen H. Department of Ophthalmology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany aut Mosel F. Institute of Microbiology, University of Duisburg-Essen, Essen, Germany aut Steuhl K.-P Department of Ophthalmology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany aut Meller D. Department of Ophthalmology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 343-350 1389-9333 16:3<343 2015 16 10561 https://doi.org/10.1007/s10561-014-9477-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9477-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thomasen H. Department of Ophthalmology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany aut NATIONALLICENCE 700 1- Mosel F. Institute of Microbiology, University of Duisburg-Essen, Essen, Germany aut NATIONALLICENCE 700 1- Steuhl K.-P Department of Ophthalmology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany aut NATIONALLICENCE 700 1- Meller D. Department of Ophthalmology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 343-350 1389-9333 16:3<343 2015 16 10561