caa a22 4500 606154582 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9486-1 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9486-1 Approaches to preserve human osteochondral allografts [Elektronische Daten] [Eduardo de Sousa, Diego Aguiar, José Barcelos, Maria Duarte, Beni Olej] Osteochondral defects may progress to osteoarthritis. Many attempts have been developed to overcome this issue, including osteochondral autografts and allografts. The goal of this study was to develop a new protocol for storage of human osteochondral allografts. Osteochondral plugs were randomly allocated in the following groups: control, immediate freezing up to −70°C, cooling at 4°C, and storage at 37°C. Samples from the cooling at 4°C and storage at 37°C groups were stored in tubes containing medium plus human albumin and analyzed after 1, 3, and 14days. The frozen groups' samples were cryopreserved for 1year in cryotubes containing medium only (FM), medium plus human albumin (FA), and medium plus human albumin and glucose (FG) and were then analyzed. Analysis involved histological study with hematoxylin-eosin and Safranin O and a modified Live/Dead assay. In samples stored both at 37 and 4°C, analysis showed statistically significant higher cellular mortality at 14days compared to 1 and 3days, but mortality in the 4°C group was lower. In the freezing protocols, the FA group showed less cellular mortality than the FM and FG groups. Cooling at 4°C offers better preservation capacity than storage at 37°C, but both offer the capacity for preservation for 14days. Adding human albumin to the storage medium is useful in reducing cellular mortality in samples frozen for 1year. Springer Science+Business Media Dordrecht, 2014 Chondrocyte nationallicence Cryopreservation nationallicence Osteochondral allografts nationallicence Cartilage nationallicence de Sousa Eduardo Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil, 500 - 5° Andar, 20940-070, Rio de Janeiro, RJ, Brazil aut Aguiar Diego Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil, 500 - 5° Andar, 20940-070, Rio de Janeiro, RJ, Brazil aut Barcelos José Amazonas Federal University, Manaus, AM, Brazil aut Duarte Maria Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil, 500 - 5° Andar, 20940-070, Rio de Janeiro, RJ, Brazil aut Olej Beni Cell Therapy Center, Unit of Clinical Research, Fluminense Federal University, Niterói, RJ, Brazil aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 425-431 1389-9333 16:3<425 2015 16 10561 https://doi.org/10.1007/s10561-014-9486-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9486-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Sousa Eduardo Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil, 500 - 5° Andar, 20940-070, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Aguiar Diego Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil, 500 - 5° Andar, 20940-070, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Barcelos José Amazonas Federal University, Manaus, AM, Brazil aut NATIONALLICENCE 700 1- Duarte Maria Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil, 500 - 5° Andar, 20940-070, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Olej Beni Cell Therapy Center, Unit of Clinical Research, Fluminense Federal University, Niterói, RJ, Brazil aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 425-431 1389-9333 16:3<425 2015 16 10561