caa a22 4500 606154590 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9492-3 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9492-3 Meniscal allograft sterilisation: effect on biomechanical and histological properties [Elektronische Daten] [David Bui, Vedran Lovric, Rema Oliver, Nicky Bertollo, David Broe, William Walsh] Sterilisation of allografts are a crucial step in ensuring safety and viability. Current sterilisation standards such as 25kGy gamma irradiation (γ) can have adverse effects on the ultrastructure and biomechanical properties of allograft tissue. Supercritical CO2 (SCCO2) technology, represents an improved sterilisation process that potentially preserves tissue properties. This study aimed to test the effect of SCCO2 sterilisation on the biomechanical and histological properties of the meniscus and compare this to the current standard of γ. Thirty-two 18-month old ovine menisci were randomly assigned into three groups for sterilisation (SCCO2, γ and control). After treatment, biomechanical indentation testing (stiffness and stress relaxation) or histological analysis [percentage of void, cells and extracellular matrix (ECM) per slide] was undertaken. Both SCCO2 and gamma groups displayed an increase in stiffness and stress relaxation as compared to control, however, this difference was lesser in samples treated with SCCO2. No significant histological quantitative differences were detected between SCCO2 and control specimens. Gamma-treated samples demonstrated a significant increase in void and decrease in ECM. Interestingly, both treatment groups demonstrated a decreasing mean void and increasing ECM percentage when analysed from outer to inner zones. No significant differences were detected in all-endpoints when analysed by section. SCCO2 sterilisation represents a potential feasible alternative to existing sterilization techniques such as γ. Springer Science+Business Media Dordrecht, 2015 Meniscus nationallicence Allograft nationallicence Supercritical carbon dioxide nationallicence Gamma irradiation nationallicence Sterilisation and Biomechanical nationallicence Bui David Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut Lovric Vedran Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut Oliver Rema Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut Bertollo Nicky Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut Broe David Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut Walsh William Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 467-475 1389-9333 16:3<467 2015 16 10561 https://doi.org/10.1007/s10561-014-9492-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9492-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bui David Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Lovric Vedran Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Oliver Rema Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Bertollo Nicky Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Broe David Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Walsh William Surgical and Orthopaedic Research Laboratories Prince of Wales Clinical School, Prince of Wales Hospital, University of New South Wales, Level 1 Clinical Sciences Building, Avoca St Randwick, 2031, Sydney, NSW, Australia aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 467-475 1389-9333 16:3<467 2015 16 10561