caa a22 4500 606154604 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-015-9493-x doi (NATIONALLICENCE)springer-10.1007/s10561-015-9493-x Potential of 5-azacytidine induction decidual stromal cells from maternal human term placenta towards cardiomyocyte-like cells in serum-free medium [Elektronische Daten] [Gecai Chen, Aihuan Yue, Zhongbao Ruan, Yigang Yin, Ruzhu Wang, Yin Ren, Li Zhu] Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. We isolated DSCs from placenta by tissue adherence. The morphology, immunophenotype, and multi-lineage potential were analyzed. Karyotype analysis (G-band) was performed to determine the source and karyotype stability of DSCs. DSCs were induced by 5-azacytidine. Expression of Myf5, α-cardiac actin, Cardiac troponin T (cTnT) and GAPDH was assessed by PCR, and cTnT expression was also analyzed by immunofluorescence. Karyotype analyses indicated that cells were derived from the maternal matrix. After induction with 5-azacytidine, DSCs expressed the cardiac-specific markers Myf5, myogenin and cTnT, indicating differentiation towards cardiomyocyte-like cells. Springer Science+Business Media Dordrecht, 2015 Decidual stromal cells (DSCs) nationallicence 5-Azacytidine nationallicence Cardiomyocyte-like cells nationallicence Chen Gecai Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut Yue Aihuan Stem Cell Research Center, Taizhou, Jiangsu Province, China aut Ruan Zhongbao Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut Yin Yigang Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut Wang Ruzhu Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut Ren Yin Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut Zhu Li Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 477-485 1389-9333 16:3<477 2015 16 10561 https://doi.org/10.1007/s10561-015-9493-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9493-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Gecai Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 700 1- Yue Aihuan Stem Cell Research Center, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 700 1- Ruan Zhongbao Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 700 1- Yin Yigang Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 700 1- Wang Ruzhu Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 700 1- Ren Yin Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 700 1- Zhu Li Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 477-485 1389-9333 16:3<477 2015 16 10561