caa a22 4500 606154612 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9483-4 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9483-4 The effect of gestational diabetes on proliferation capacity and viability of human umbilical cord-derived stromal cells [Elektronische Daten] [Nadia Wajid, Rashida Naseem, Sanam Anwar, Sana Awan, Muhammad Ali, Sara Javed, Fatima Ali] Stomal cells derived from Wharton's jelly of human umbilical cord (WJMSCs) are considered as the potential therapeutic agents for regeneration and are getting famous for stem cell banking. Our study aims to evaluate the effects of gestational diabetes on proliferation capacity and viability of WJMSCs. Mesenchymal stromal cells were isolated from Wharton's jelly of human umbilical cords from normal and gestational diabetic (DWJMSCs) mothers. Growth patterns of both types of cells were analyzed through MTT assay and population doubling time. Cell survival, cell death and glucose utilization were estimated through trypan blue exclusion assay, LDH assay and glucose detection assay respectively. Angiogenic ability was evaluated by immunocytochemistry and ELISA for VEGF A. Anti-cancerous potential was analyzed on HeLa cells. DWJMSCs exhibited low proliferative rate, increased population doubling time, reduced cell viability and increased cell death. Interestingly, DWJMSCs were found to have a reduced glucose utilization and anti-cancerous ability while enhanced angiogenic ability. Gestational diabetes induces adverse effects on growth, angiogenic and anti-cancerous potential of WJMSCs. Springer Science+Business Media Dordrecht, 2014 Diabetes nationallicence Gestation nationallicence Stem cells nationallicence Wharton's jelly nationallicence Mesenchymal stem cells nationallicence Wajid Nadia IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Naseem Rashida IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Anwar Sanam IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Awan Sana IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Ali Muhammad IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Javed Sara IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Ali Fatima IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 389-397 1389-9333 16:3<389 2015 16 10561 https://doi.org/10.1007/s10561-014-9483-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9483-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wajid Nadia IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 700 1- Naseem Rashida IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 700 1- Anwar Sanam IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 700 1- Awan Sana IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 700 1- Ali Muhammad IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 700 1- Javed Sara IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 700 1- Ali Fatima IMBB, The University of Lahore, Defense Road Campus, Lahore, Pakistan aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 389-397 1389-9333 16:3<389 2015 16 10561