caa a22 4500 606154647 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9482-5 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9482-5 Efficacy of decontamination protocol by antimicrobial treatment in Iranian Tissue Bank (ITB) [Elektronische Daten] [Simin Dashti-Khavidaki, Fariba Dabardani, Mitra Mahdavi-Mazdeh, Elham Ravanasa, Seyed Hosseini] Iranian Tissue Bank established in 1994 provides soft tissues for implantation in Iran. This study was designed to evaluate the efficacy of decontamination process of cardiac and soft tissues in Iranian Tissue Bank. In this bank after initial assessments, the tissues were incubated in a 5-antibiotic cocktail at room temperature for 24h and then at 4°C for 14days. Contamination status was compared before and after antibiotic cocktail incubation. Of 3,315 assessed tissues, 1,057 were pericardia, 1,051 were fascia and 1,207 were other soft tissues including tibialis and aorta. The initial contamination rate was 36.86%. Pericardia showed the highest contamination rate. Klebsiella species was the most prevalent organism causing contamination. Decontamination rate after antibiotic incubation was 86.91% with the highest successful decontamination rate for fascia tissue. Klebsiella species was the major source of contamination in tissues that remained contaminated after antibiotic incubation. This may be due to resistance of this organism to applied antibiotics in the decontamination cocktail possibly due to a negative drug interaction between aminoglycoside and penicillin derivatives in this antibiotic cocktail. In conclusion collected data shows comparable efficacy of the decontamination process that is used in ITB compared with homograft banks of other countries. Springer Science+Business Media Dordrecht, 2014 Antibiotic nationallicence Cardiac tissues nationallicence Decontamination nationallicence Donated tissues nationallicence Tissue bank nationallicence Soft tissues nationallicence Dashti-Khavidaki Simin Nephrology Research Center, Tehran University of Medical Sciences, Tehran, Iran aut Dabardani Fariba Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran aut Mahdavi-Mazdeh Mitra Nephrology Research Center, Tehran University of Medical Sciences, Tehran, Iran aut Ravanasa Elham Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran aut Hosseini Seyed Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 381-388 1389-9333 16:3<381 2015 16 10561 https://doi.org/10.1007/s10561-014-9482-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9482-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dashti-Khavidaki Simin Nephrology Research Center, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Dabardani Fariba Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Mahdavi-Mazdeh Mitra Nephrology Research Center, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Ravanasa Elham Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Hosseini Seyed Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 381-388 1389-9333 16:3<381 2015 16 10561