caa a22 4500 606154663 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9478-1 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9478-1 Technical note: comparison of the PrestoBlue and LDH release assays with the MTT assay for skin viability assessment [Elektronische Daten] [Sonia Gaucher, Mohamed Jarraya] MTT assay is the gold standard for assessing skin sample viability but it is time-consuming. Here we compared the MTT test with two other assays for the assessment of skin viability. The MTT, PrestoBlue (colorimetric method) and LDH release assays were applied to fresh and cryopreserved skin. Skin viability was considered proportional to the optical density values of the relevant analytes. PrestoBlue did not reliably distinguish between fresh and cryopreserved skin. The LDH release assay did not allow us to establish a viability index. We recommend the MTT assay for assessing skin viability. Springer Science+Business Media Dordrecht, 2014 Skin viability nationallicence MTT nationallicence PrestoBlue nationallicence LDH release nationallicence Optical density nationallicence Gaucher Sonia Faculté de Médecine, Université Paris Descartes, Paris, France aut Jarraya Mohamed Banque de Tissus Humains, Hôpitaux Universitaires Saint-Louis Lariboisière, Site Saint Louis, AP-HP, Paris, France aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 325-329 1389-9333 16:3<325 2015 16 10561 https://doi.org/10.1007/s10561-014-9478-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9478-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gaucher Sonia Faculté de Médecine, Université Paris Descartes, Paris, France aut NATIONALLICENCE 700 1- Jarraya Mohamed Banque de Tissus Humains, Hôpitaux Universitaires Saint-Louis Lariboisière, Site Saint Louis, AP-HP, Paris, France aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 325-329 1389-9333 16:3<325 2015 16 10561