caa a22 4500 606154698 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10561-014-9488-z doi (NATIONALLICENCE)springer-10.1007/s10561-014-9488-z Negative effect of combined cysteine and glutathione in soy lecithin-based extender on post-thawed ram spermatozoa [Elektronische Daten] [Mahdi Zhandi, Mohsen Sharafi] This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n=4, one ejaculate for each ram) were pooled and divided into three equal parts, and each part was diluted with one of following extender: (1) soy lecithin-based extender containing no cysteine and no glutathione (C0-G0), (2) soy lecithin-based extender containing cysteine (5mM) and glutathione (5mM) (C5-G5), and (3) soy lecithin-based extender containing cysteine (10mM) and glutathione (10mM) (C10-G10). After freeze-thawing process, motility and velocity parameters, plasma membrane integrity and functionality, mitochondrial activity, and apoptosis features of spermatozoa were evaluated. The obtained results showed that total and progressive motility, plasma membrane integrity and functionality, and live post-thawed spermatozoa was lower in C10-G10 extender compared to C0-G0 and C5-G5 extenders (P<0.05). Also, the percentage of dead spermatozoa was higher in C10-G10 extender compared to C0-G0 extender (P<0.05). Apoptotic spermatozoa was lower in C10-G10 extender compared to C0-G0 and C5-G5 extenders (P<0.05). All velocity parameters, exception of BCF, did not different between extenders (P>0.05). In conclusion, it seems that high concentration of combined cysteine and glutathione in soy lecithin-based semen extender has a detrimental effect of post-thawed ram sperm quality. Springer Science+Business Media Dordrecht, 2014 Cysteine nationallicence Freezing nationallicence Glutathione nationallicence Ram nationallicence Sperm nationallicence Zhandi Mahdi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran aut Sharafi Mohsen Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran aut Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 443-448 1389-9333 16:3<443 2015 16 10561 https://doi.org/10.1007/s10561-014-9488-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9488-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhandi Mahdi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Sharafi Mohsen Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/3(2015-09-01), 443-448 1389-9333 16:3<443 2015 16 10561