caa a22 4500 60615471X CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9498-5 doi (NATIONALLICENCE)springer-10.1007/s10561-015-9498-5 Effect of uncontrolled freezing on biological characteristics of human dental pulp stem cells [Elektronische Daten] [Ajay Kumar, Shalmoli Bhattacharyya, Vidya Rattan] Human dental pulp stem cells (hDPSCs) hold great promise as a source of adult stem cells for utilization in regenerative medicine. Successful storage and post thaw recovery of DPSCs without loss of function is a key issue for future clinical application. Most of the cryopreservation methods use controlled rate freezing and vapor phase nitrogen to store stem cells. But these methods are both expensive and laborious. In this study, we isolated DPSCs from a patient undergoing impacted mandibular third molar extraction. We adopted eight different methods of cryopreservation at −80°C for long term storage of the DPSC aliquots. Various parameters like proliferation, cell death, cell cycle, retention of stemness markers and differentiation potential were studied post cryopreservation period of 1year. We observed successful recovery of stem cells in every method and a significant difference in proliferation potential and cell death between samples stored by different methods. However, post thaw, all cells retained their stemness markers. All DPSCs stored by different methods were able to differentiate into osteoblast like cells, adipocytes and neural cells. Based on these parameters we concluded that uncontrolled freezing at a temperature of −80°C is as effective as controlled freezing using ethanol vessels and other cryopreservation methods. To the best of our knowledge, our study provides the first proof of concept that long term storage in uncontrolled freezing of cells at −80°C in 10% DMSO does not affect the revival capacity of hDPSCs. This implies that DPSCs may be used successfully for tissue engineering and cell based therapeutics even after long term, uncontrolled cryopreservation. Springer Science+Business Media Dordrecht, 2015 Dental pulp stem cells nationallicence Cryopreservation nationallicence Differentiation nationallicence Kumar Ajay Department of Biophysics, PGIMER, 160012, Chandigarh, India aut Bhattacharyya Shalmoli Department of Biophysics, PGIMER, 160012, Chandigarh, India aut Rattan Vidya Unit of Oral and Maxillofacial Surgery, Oral Health Science Centre, PGIMER, Chandigarh, India aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 513-522 1389-9333 16:4<513 2015 16 10561 https://doi.org/10.1007/s10561-015-9498-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9498-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar Ajay Department of Biophysics, PGIMER, 160012, Chandigarh, India aut NATIONALLICENCE 700 1- Bhattacharyya Shalmoli Department of Biophysics, PGIMER, 160012, Chandigarh, India aut NATIONALLICENCE 700 1- Rattan Vidya Unit of Oral and Maxillofacial Surgery, Oral Health Science Centre, PGIMER, Chandigarh, India aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 513-522 1389-9333 16:4<513 2015 16 10561