caa a22 4500 606154728 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9500-2 doi (NATIONALLICENCE)springer-10.1007/s10561-015-9500-2 Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity? [Elektronische Daten] [Francis Smit, Dreyer Bester, Johannes van den Heever, Franziska Schlegel, Lezelle Botes, Pascal Dohmen] This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24h, n=10); group 2 (48h, n=10); group 3 (72h, n=10). In each group, 5 CPAs were studied in vitro after cryopreservation and thawing. The other 5 CPAs were implanted in juvenile sheep for a minimum of 180days. Serology samples were obtained and echocardiography was performed before euthanasia. Hematoxylin and eosin (H&E), scanning electron microscopy (SEM), von Kossa, Picrosirius red, α-actin, immunohistochemistry [von Willebrand factor (vWF), CD4, CD31 and CD34] and calcium content analyses were performed on explanted CPAs. The in vitro and in vivo studies failed to demonstrate any change in tensile strength, Young's Modulus and thermal denaturation (Td) results between the groups. SEM demonstrated a reduction in endothelial cells (50% at 24h, 60.9% at 48h and 40.9% at 72h), but H&E could not demonstrate autolysis in any CPA in vitro. All cultures were negative. In the explanted groups, IgE, IgM and IgG results were inconclusive. Echocardiography demonstrated normal valve function in all groups. H&E and Picrosirius red staining confirmed tissue integrity. vWF, CD31 and CD34 staining confirmed a monolayer of endothelial cells in all explanted valves. Calcium content of explanted CPA leaflets was similar. This experimental study supports the concept of prolonging the cold ischemic harvesting time of cryopreserved homografts to reduce homograft shortage. Springer Science+Business Media Dordrecht, 2015 Cryopreserved homografts nationallicence Warm ischemic time nationallicence Right ventricular outflow tract reconstruction nationallicence Juvenile sheep model nationallicence Smit Francis Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut Bester Dreyer Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut van den Heever Johannes Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut Schlegel Franziska Department of Cardiac Surgery, Heart Centre Leipzig, University of Leipzig, Leipzig, Saxony, Germany aut Botes Lezelle Department of Health Sciences, Central University of Technology (CUT), Free State, Private Bag X20539, Bloemfontein, South Africa aut Dohmen Pascal Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 531-544 1389-9333 16:4<531 2015 16 10561 https://doi.org/10.1007/s10561-015-9500-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9500-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smit Francis Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut NATIONALLICENCE 700 1- Bester Dreyer Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut NATIONALLICENCE 700 1- van den Heever Johannes Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut NATIONALLICENCE 700 1- Schlegel Franziska Department of Cardiac Surgery, Heart Centre Leipzig, University of Leipzig, Leipzig, Saxony, Germany aut NATIONALLICENCE 700 1- Botes Lezelle Department of Health Sciences, Central University of Technology (CUT), Free State, Private Bag X20539, Bloemfontein, South Africa aut NATIONALLICENCE 700 1- Dohmen Pascal Department Cardiothoracic Surgery, Faculty of Health Sciences, University of the Free State (UFS), Bloemfontein, South Africa aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 531-544 1389-9333 16:4<531 2015 16 10561