caa a22 4500 606154736 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9495-8 doi (NATIONALLICENCE)springer-10.1007/s10561-015-9495-8 Effect of combination of nerve fragments with nerve growth factor in autologous epineurium small gap coaptation on peripheral nerve injury repair [Elektronische Daten] [Bo Feng, Hua Ma, He Hu, Lan Zhang, Zhi Zhang, Youming Pang, Yongjun Wang, Kecheng Niu, Ligong Lin] The aim of this study was to explore the effect of the combination of nerve fragments with nerve growth factor (NGF) on the repair of peripheral nerve injury through autologous epineurium small gap coaptation. A total of 150 male Sprague-Dawley rats weighing 200-250g were divided into five groups randomly with 30 rats per group, including the following: a control group that was subjected to traditional end-to-end neuroanastomosis; an autologous epineurium small gap group that received autologous epineurium small gap coaptation suture; a nerve fragments group in which nerve fragments were added to the small gap; an NGF group in which NGF was added to the small gap; and an NGF combined with nerve fragments group in which both NGF and nerve fragments were added to the small gap. All groups were examined at 4, 6, and 8weeks after the operation, respectively; furthermore, electroneurophysiological detection and histological observation were performed at 8weeks. Autonomic activities and root ulcers recovered sooner in rats in the NGF combined with nerve fragments group than the other groups. Moreover, the numbers of regenerated nerve fibers were greater and nerve conduction velocity was faster in the NGF combined with nerve fragments group than the other groups. Therefore, the combination of NGF with nerve fragments plays a significant role in the repair of peripheral nerve injury through autologous epineurium small gap coaptation. Therefore, compared with the other four methods, the combination of nerve fragments with NGF on the repair of peripheral nerve injury through autologous epineurium small gap coaptation has a better effect. Springer Science+Business Media Dordrecht, 2015 Nerve repair nationallicence Small gap bridging nationallicence Nerve debris nationallicence Nerve growth factor nationallicence Feng Bo Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Ma Hua Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Hu He Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Zhang Lan Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Zhang Zhi Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Pang Youming Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Wang Yongjun Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Niu Kecheng Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Lin Ligong Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 497-502 1389-9333 16:4<497 2015 16 10561 https://doi.org/10.1007/s10561-015-9495-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9495-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Bo Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Ma Hua Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Hu He Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Zhang Lan Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Zhang Zhi Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Pang Youming Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Wang Yongjun Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Niu Kecheng Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 700 1- Lin Ligong Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, 014010, Baotou, Inner Mongolia, China aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 497-502 1389-9333 16:4<497 2015 16 10561