caa a22 4500 606154752 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9503-z doi (NATIONALLICENCE)springer-10.1007/s10561-015-9503-z Human dental pulp stem cells cultured onto dentin derived scaffold can regenerate dentin-like tissue in vivo [Elektronische Daten] [Ha Tran, Vu Doan] Regeneration of dentin tissues in the pulp space of teeth serves the ultimate goal of preserving teeth via endodontic approaches. In recent times, many studies suggested that human dentin scaffolds combined with dental stem cells was a potential strategy for the complete dentin tissue regeneration. In this study, human dental pulp stem cells (DPSCs) were isolated and cultured. Dentin specimens were prepared from human third molars and treated with ethylene diamine tetra-acetic acid and citric acid to remove the smear layer. Then, DPSCs were cultured onto human treated dentin (hTD) and implanted in mouse model for 4, 6 and 8weeks. The resulting grafts were assessed by hematoxylin and eosin stain and immunohistochemical stains. As a result, DPSCs were supported and induced to regenerate of dentin-like tissues which expressed specific dentin markers such as dentin sialophosphoprotein and dentin matrix protein 1 by combination with hTD in vivo. Furthermore, cells existed in the newly-formed dentin-like tissues also expressed typical human mitochondria antibodies, demonstrated that new tissues originated from human. In conclusion, the obtain results extend hopefully newly-established therapy to apply in endodontics and traumatic dental hard tissues. Springer Science+Business Media Dordrecht, 2015 Dentin nationallicence Dental pulp nationallicence Tissue engineering nationallicence Mesenchymal stem cells nationallicence Extracellular matrix protein nationallicence Tran Ha Department of Physiology and Animal Biotechnology, Faculty of Biology, University of Science, Vietnam National University at Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam aut Doan Vu Department of Physiology and Animal Biotechnology, Faculty of Biology, University of Science, Vietnam National University at Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 559-568 1389-9333 16:4<559 2015 16 10561 https://doi.org/10.1007/s10561-015-9503-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9503-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tran Ha Department of Physiology and Animal Biotechnology, Faculty of Biology, University of Science, Vietnam National University at Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam aut NATIONALLICENCE 700 1- Doan Vu Department of Physiology and Animal Biotechnology, Faculty of Biology, University of Science, Vietnam National University at Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 559-568 1389-9333 16:4<559 2015 16 10561