caa a22 4500 606154795 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9494-9 doi (NATIONALLICENCE)springer-10.1007/s10561-015-9494-9 Comparison of human dermal fibroblasts (HDFs) growth rate in culture media supplemented with or without basic fibroblast growth factor (bFGF) [Elektronische Daten] [Narges Abdian, Payam Ghasemi-Dehkordi, Morteza Hashemzadeh-Chaleshtori, Mahbobe Ganji-Arjenaki, Abbas Doosti, Beheshteh Amiri] Basic fibroblast growth factor (bFGF or FGF-2) is a member of the FGF family secreted by different kinds of cells like HDFs and it is an important nutritional factor for cell growth and differentiation. The HDFs release bFGF in culture media at very low. The present study aims to investigate the HDFs growth rate in culture media supplemented either with or without bFGF. In brief, HDFs were isolated from human foreskin sample and were cultured in vitro in media containing bFGF and lack of this factor. The cells growth rate was calculated by trypan blue. The karyotyping was performed using G-banding to investigate the chromosomal abnormality of HDFs in both groups. Total RNA of each groups were extracted and cDNA samples were synthesized then, real-time Q-PCR was used to measure the expression level of p27kip1 and cyclin D1 genes normalized to internal control gene (GAPDH). The karyotype analysis showed that HDFs cultured in media or without bFGF had normal karyotype (46 chromosomes, XY) and chromosomal abnormalities were not observed. The cell growth rates in both groups were normal with proliferated exponentially but the slope of growth curve in HDFs cultured in media containing bFGF was increased. Karyotyp test showed that bFGF does not affect on cytogenetic stability of cells. The survey of p27kip1 and cyclin D1 genes by real-time Q-PCR showed that the expression level of these genes were up-regulated when adding bFGF in culture media (p<0.05). The findings of the present study demonstrate that appropriate supplementation of culture media with growth factor like bFGF could enhance the proliferation and differentiation capacity of cells and improve cells growth rate. Similarly, fibroblast growth factors did not induce any chromosomal abnormality in cells. Furthermore, in HDFs cultured in bFGF supplemented media, the p27kip1 and cyclin D1 genes were up-regulated and suggesting an important role for bFGF in cell-cycle regulation and progression and fibroblast division stimulation. It also suggests that the effects of bFGF on different cell types with/or without production of bFGF or other regulation factors be investigated in future. Springer Science+Business Media Dordrecht, 2015 HDF nationallicence bFGF nationallicence Cell growth rate nationallicence Karyotyping nationallicence Real-time PCR nationallicence HDFs : Human dermal fibroblasts nationallicence bFGF : Basic fibroblast growth factor nationallicence Abdian Narges Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut Ghasemi-Dehkordi Payam Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut Hashemzadeh-Chaleshtori Morteza Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut Ganji-Arjenaki Mahbobe Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran aut Doosti Abbas Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran aut Amiri Beheshteh Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 487-495 1389-9333 16:4<487 2015 16 10561 https://doi.org/10.1007/s10561-015-9494-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9494-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abdian Narges Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut NATIONALLICENCE 700 1- Ghasemi-Dehkordi Payam Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut NATIONALLICENCE 700 1- Hashemzadeh-Chaleshtori Morteza Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut NATIONALLICENCE 700 1- Ganji-Arjenaki Mahbobe Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran aut NATIONALLICENCE 700 1- Doosti Abbas Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran aut NATIONALLICENCE 700 1- Amiri Beheshteh Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, 8813833435, Shahrekord, Iran aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 487-495 1389-9333 16:4<487 2015 16 10561