caa a22 4500 606154817 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9504-y doi (NATIONALLICENCE)springer-10.1007/s10561-015-9504-y Development of an improved bone washing and demineralisation process to produce large demineralised human cancellous bone sponges [Elektronische Daten] [Mark Eagle, Paul Rooney, John Kearney] Shaped demineralised bone matrices (DBM) made from cancellous bone have important uses in orthopaedic and dental procedures, where the properties of the material allow its insertion into confined defects, therefore acting as a void filler and scaffold onto which new bone can form. The sponges are often small in size, <1.0cm3. In this study, we report on an improved bone washing and demineralisation process that allows production of larger DBM sponges (3.375 or 8.0cm3) from deceased donor bone. These sponges were taken through a series of warm water washes, some with sonication, centrifugation, 100% ethanol and two decontamination chemical washes and optimally demineralised using 0.5N hydrochloric acid under vacuum. Demineralisation was confirmed by quantitative measurement of calcium and qualitatively by compression. Protein and DNA removal was also determined. The DBM sponges were freeze dried before terminal sterilisation with a target dose of 25kGy gamma irradiation whilst frozen. Samples of the sponges were examined histologically for calcium, collagen and the presence of cells. The data indicated lack of cells, absence of bone marrow and a maximum of 1.5% residual calcium. Springer Science+Business Media Dordrecht, 2015 Demineralised bone sponge nationallicence Vacuum nationallicence Protein nationallicence DNA nationallicence Allograft nationallicence Eagle Mark NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, L24 8RB, Liverpool, UK aut Rooney Paul NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, L24 8RB, Liverpool, UK aut Kearney John NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, L24 8RB, Liverpool, UK aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 569-578 1389-9333 16:4<569 2015 16 10561 https://doi.org/10.1007/s10561-015-9504-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9504-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eagle Mark NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, L24 8RB, Liverpool, UK aut NATIONALLICENCE 700 1- Rooney Paul NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, L24 8RB, Liverpool, UK aut NATIONALLICENCE 700 1- Kearney John NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, L24 8RB, Liverpool, UK aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 569-578 1389-9333 16:4<569 2015 16 10561