caa a22 4500 606154825 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9513-x doi (NATIONALLICENCE)springer-10.1007/s10561-015-9513-x Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells [Elektronische Daten] [Ivan Varga, Michal Miko, Lenka Oravcová, Tatiana Bačkayová, Ján Koller, Ľuboš Danišovič] White adipose tissue was long perceived as a passive lipid storage depot but it is now considered as an active and important endocrine organ. It also harbours not only adipocytes and vascular cells but also a wide array of immunologically active cells, including macrophages and lymphocytes, which may induce obesity-related inflammation. Recently, adipose tissue has been reported as a source of adult mesenchymal stem cells with wide use in regenerative medicine and tissue engineering. Their relatively non-complicated procurement and collection (often performed as liposuction during aesthetic surgery) and grand plasticity support this idea even more. We focused our research on exploring the issues of isolation and long-term cultivation of mesenchymal stem cells obtained from adipose tissue. Ultra-structural morphology of the cells cultivated in vitro has been studied and analysed in several cultivation time periods and following serial passages—up to 30 passages. In the first passages they had ultra-structural characteristics of cells with high proteosynthetic activity. Within the cytoplasm, big number of small lipid droplets and between them, sparsely placed, small and inconspicuous, electron-dense, lamellar bodies, which resembled myelin figures were observed. The cells from the later passages contained high number of lamellar electron-dense structures, which filled out almost the entire cytoplasm. In between, mitochondria were often found. These bodies were sometimes small and resembled myelin figures, but several of them reached huge dimensions (more than 1µm) and their lamellar structure was not distinguishable. We did not have an answer to the question about their function, but they probably represented the evidence of active metabolism of lipids present in the cytoplasm of these cells or represented residual bodies, which arise after the breakdown of cellular organelles, notably mitochondria during long-term cultivation. Springer Science+Business Media Dordrecht, 2015 Adipose tissue-derived stem cells nationallicence Isolation nationallicence Long-time cultivation nationallicence Transmission electron microscopy nationallicence Flow cytometry analysis nationallicence Varga Ivan Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut Miko Michal Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut Oravcová Lenka Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut Bačkayová Tatiana Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia aut Koller Ján Department of Burns and Reconstruction Surgery, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut Danišovič Ľuboš Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 639-647 1389-9333 16:4<639 2015 16 10561 https://doi.org/10.1007/s10561-015-9513-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9513-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Varga Ivan Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Miko Michal Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Oravcová Lenka Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Bačkayová Tatiana Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Koller Ján Department of Burns and Reconstruction Surgery, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Danišovič Ľuboš Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 639-647 1389-9333 16:4<639 2015 16 10561