caa a22 4500 606154892 CHVBK 20210128100600.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9508-7 doi (NATIONALLICENCE)springer-10.1007/s10561-015-9508-7 Effect of dynamic seeding methods on the distribution of fibroblasts within human acellular dermis [Elektronische Daten] [Mario Vitacolonna, Djeda Belharazem, Peter Hohenberger, Eric Roessner] The purpose of this investigation was to compare different dynamic cell seeding methods regarding their seeding efficiency, homogeneity, infiltration depth and proliferation within a human acellular dermis. In addition, the growth behaviour was observed during a 12-day static in vitro culture. The dynamic methods included orbital-shaker seeding and the use of a plate centrifuge with different rotational speeds, combinations of low-pressure for matrix degassing and centrifugal seeding. Scaffolds were incubated for up to 12days statically. Cell distribution and infiltration depth were analysed histologically at days 0, 4, 8 and 12. Seeding efficiency and cell proliferation were quantified with the MTT-assay at the same time points. Centrifugal seeding with 300g for 5×1min combined with matrix degassing significantly increased the seeding efficiency and homogeneity compared to the other methods. However, following static culture, no cells were detectable after 4days in the inner matrix zones. Furthermore, none of the degassing+centrifugation groups reached a significantly higher proliferation at day 8 compared to the reference. The use of a single dynamic method resulted in an inefficient cell seeding. We archived the highest seeding efficiency, homogeneity and infiltration depth using a combination of degassing+centrifugation at 300g for 5×1min. Springer Science+Business Media Dordrecht, 2015 Tissue-Engineering nationallicence Cell seeding nationallicence Human acellular dermis nationallicence Dynamic cell seeding methods nationallicence Scaffold seeding nationallicence Comparison of dynamic seeding methods nationallicence Vitacolonna Mario Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, University of Heidelberg, Theodor- Kutzer- Ufer 1-3, 68167, Mannheim, Germany aut Belharazem Djeda Institute of Pathology, University Medical Centre Mannheim, University of Heidelberg, 68167, Mannheim, Germany aut Hohenberger Peter Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, University of Heidelberg, Theodor- Kutzer- Ufer 1-3, 68167, Mannheim, Germany aut Roessner Eric Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, University of Heidelberg, Theodor- Kutzer- Ufer 1-3, 68167, Mannheim, Germany aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 605-614 1389-9333 16:4<605 2015 16 10561 https://doi.org/10.1007/s10561-015-9508-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9508-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vitacolonna Mario Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, University of Heidelberg, Theodor- Kutzer- Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Belharazem Djeda Institute of Pathology, University Medical Centre Mannheim, University of Heidelberg, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Hohenberger Peter Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, University of Heidelberg, Theodor- Kutzer- Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 700 1- Roessner Eric Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, University of Heidelberg, Theodor- Kutzer- Ufer 1-3, 68167, Mannheim, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 605-614 1389-9333 16:4<605 2015 16 10561