caa a22 4500 606154906 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10561-015-9506-9 doi (NATIONALLICENCE)springer-10.1007/s10561-015-9506-9 The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality [Elektronische Daten] [Mahmood Amini, Hamid Kohram, Ahmad Zare Shahaneh, Mahdi Zhandi, Hossein Sharideh, Mohammad Nabi] Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reason of declined motility and fertility of sperm during the freeze-thawing process. This study was conducted to determine the influence of vitamin C and vitamin E on rooster post-thawed sperm motility, viability and malondialdehyde (MDA) level. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing with no antioxidants (control), or containing 100 (C100), 200 (C200), 400 (C400), 800 (C800) µg/mL vitamin C, and 2 (E2), 5 (E5), 10 (E10) and 15 (E15) µg/mL vitamin E. After thawing, total and progressive sperm motility, sperm viability and semen MDA level were assessed. The results shown that C200 and E5 extenders resulted in higher total motility (p<0.05) compared to other extenders, with exception of E10 extender. Progressive motility was higher in E5 extender (p<0.05) compared to other extenders, with exception of C200 and E10 extenders. Also, C200 and E5 extenders resulted in higher viability of post-thawed spermatozoa (p<0.05) compared to other extenders. Finally, the results showed that MDA level was lower in C100 and C200 extenders compared to other extenders (p<0.05), with exception of E5 extender. In conclusion, the results of the present study demonstrate that C200 and E5 can improve the function of post-thawed rooster spermatozoa. Springer Science+Business Media Dordrecht, 2015 Rooster nationallicence Semen nationallicence Vitamin E nationallicence Vitamin C nationallicence Freezing nationallicence Amini Mahmood Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut Kohram Hamid Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut Zare Shahaneh Ahmad Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut Zhandi Mahdi Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut Sharideh Hossein Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut Nabi Mohammad Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 587-592 1389-9333 16:4<587 2015 16 10561 https://doi.org/10.1007/s10561-015-9506-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-015-9506-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amini Mahmood Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Kohram Hamid Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Zare Shahaneh Ahmad Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Zhandi Mahdi Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Sharideh Hossein Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Nabi Mohammad Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/4(2015-12-01), 587-592 1389-9333 16:4<587 2015 16 10561