caa a22 4500 606154930 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10561-014-9458-5 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9458-5 Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa [Elektronische Daten] [Mohsen Sharafi, Mahdi Zhandi, Abbas Akbari Sharif] The purpose of the current study was to evaluate the effects of cysteine (C) and glutathione (G) on the post-thawed ram sperm quality. Collected semen samples from four mature rams were diluted with five soybean lecithin (SL)-based extenders containing: no antioxidant (SL-0), 5mM cysteine (SL-C5), 10mM cysteine (SL-C10), 5mM glutathione (SL-G5) and 10mM glutathione (SL-G10). After freeze-thawing process, motion and velocity parameters, plasma membrane integrity and functionality, morphological abnormality, lipid peroxidation, acrosomal status, mitochondria activity, and apoptosis status of post-thawed ram spermatozoa were assessed. The results showed that SL-C10 increased the total motility and plasma membrane integrity (p<0.05) of post-thawed ram spermatozoa (55.86±1.37 and 60.57±1.34%) compared to other extenders. Progressive motility was significantly higher in SL-C10 (24.71±1.13%) compared to SL-0 (20±1.13%) and SL-G10 (15±1.13%). Mitochondrial activity was significantly higher in SL-C10 (56.83±2.29%) compared to SL-G10 (38.75±2.29%). Capacitation and acrosomal status, lipid peroxidation, and the percentage of dead spermatozoa were not affected by different extenders. The percentage of live spermatozoa was higher in SL-C10 (56.33±1.35%) compared to other extenders. Also, SL-C10 resulted in a lower percentage of apoptotic spermatozoa (14.17±0.53%) compared to other extenders. The results of this study showed that supplementation of SL-based ram semen extender with 10mM cysteine resulted in an improved quality of post-thawed ram spermatozoa. Springer Science+Business Media Dordrecht, 2014 Cryopreservation nationallicence Cysteine nationallicence Glutathione nationallicence Ram nationallicence Semen nationallicence Sharafi Mohsen Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran aut Zhandi Mahdi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran aut Akbari Sharif Abbas Jahad-e-Keshavarzi Organization of Tehran Province, Breeding Station of Zandi Sheep, Varamin, Iran aut Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 261-269 1389-9333 16:2<261 2015 16 10561 https://doi.org/10.1007/s10561-014-9458-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9458-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharafi Mohsen Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Zhandi Mahdi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Akbari Sharif Abbas Jahad-e-Keshavarzi Organization of Tehran Province, Breeding Station of Zandi Sheep, Varamin, Iran aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 261-269 1389-9333 16:2<261 2015 16 10561