caa a22 4500 606154949 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10561-014-9467-4 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9467-4 Decellularization of human dermis using non-denaturing anionic detergent and endonuclease: a review [Elektronische Daten] [Mark Moore, Brian Samsell, Glenna Wallis, Sherry Triplett, Silvia Chen, Alyce Jones, Xiaofei Qin] Decellularized human dermis has been used for a number of clinical applications including wound healing, soft tissue reconstruction, and sports medicine procedures. A variety of methods exist to prepare this useful class of biomaterial. Here, we describe a decellularization technology (MatrACELL®) utilizing a non-denaturing anionic detergent, N-Lauroyl sarcosinate, and endonuclease, which was developed to remove potentially immunogenic material while retaining biomechanical properties. Effective decellularization was demonstrated by a residual DNA content of ≤4ng/mg of wet weight which represented >97% DNA removal compared to unprocessed dermis. Two millimeter thick MatrACELL processed human acellular dermal matrix (MH-ADM) exhibited average ultimate tensile load to failure of 635.4±199.9N and average suture retention strength of 134.9±55.1N. Using an in vivo mouse skin excisional model, MH-ADM was shown to be biocompatible and capable of supporting cellular and vascular in-growth. Finally, clinical studies of MH-ADM in variety of applications suggest it can be an appropriate scaffold for wound healing, soft tissue reconstruction, and soft tissue augmentation. The Author(s), 2014 Allograft nationallicence Homograft nationallicence Dermis nationallicence Decellularized nationallicence Acellular dermal matrix nationallicence MatrACELL nationallicence Moore Mark Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Samsell Brian Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Wallis Glenna Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Triplett Sherry Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Chen Silvia Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Jones Alyce Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Qin Xiaofei Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 249-259 1389-9333 16:2<249 2015 16 10561 https://doi.org/10.1007/s10561-014-9467-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9467-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moore Mark Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 700 1- Samsell Brian Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 700 1- Wallis Glenna Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 700 1- Triplett Sherry Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 700 1- Chen Silvia Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 700 1- Jones Alyce Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 700 1- Qin Xiaofei Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, 23453, Virginia Beach, VA, USA aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 249-259 1389-9333 16:2<249 2015 16 10561