caa a22 4500 606154957 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10561-014-9461-x doi (NATIONALLICENCE)springer-10.1007/s10561-014-9461-x High-dose electron beam sterilization of soft-tissue grafts maintains significantly improved biomechanical properties compared to standard gamma treatment [Elektronische Daten] [A. Hoburg, S. Keshlaf, T. Schmidt, M. Smith, U. Gohs, C. Perka, A. Pruss, S. Scheffler] Allografts have gained increasing popularity in anterior cruciate ligament (ACL) reconstruction. However, one of the major concerns regarding allografts is the possibility of disease transmission. Electron beam (Ebeam) and Gamma radiation have been proven to be successful in sterilization of medical products. In soft tissue sterilization high dosages of gamma irradiation have been shown to be detrimental to biomechanical properties of grafts. Therefore, it was the objective of this study to compare the biomechanical properties of human bone-patellar tendon-bone (BPTB) grafts after ebeam with standard gamma irradiation at medium (25kGy) and high doses (34kGy). We hypothesized that the biomechanical properties of Ebeam irradiated grafts would be superior to gamma irradiated grafts. Paired 10mm-wide human BPTB grafts were harvested from 20 donors split into four groups following irradiation with either gamma or Ebeam (each n=10): (A) Ebeam 25kGy, (B) Gamma 25kGy, (C) Ebeam 34kGy (D) Gamma 34kGy and ten non-irradiated BPTB grafts were used as controls. All grafts underwent biomechanical testing which included preconditioning (ten cycles, 0-20N); cyclic loading (200 cycles, 20-200N) and a load-to-failure (LTF) test. Stiffness of non-irradiated controls (199.6±59.1N/mm) and Ebeam sterilized grafts did not significantly differ (152.0±37.0N/mm; 192.8±58.0N/mm), while Gamma-irradiated grafts had significantly lower stiffness than controls at both irradiation dosages (25kGy: 126.1±45.4N/mm; 34kGy: 170.6±58.2N/mm) (p<0.05). Failure loads at 25kGy were significantly lower in the gamma group (1,009±400N), while the failure load was significantly lower in both study groups at high dose irradiation with 34kGy (Ebeam: 1,139±445N, Gamma: 1,073±617N) compared to controls (1,741±304N) (p<0.05). Creep was significantly larger in the gamma irradiated groups (25kGy: 0.96±1.34mm; 34kGy: 1.06±0.58mm) than in the Ebeam (25kGy: 0.50±0.34mm; 34kGy: 0.26±0.24mm) and control (0.20±0.18mm) group that did not differ significantly. Strain difference was not different between either control or study groups (controls: 1.0±0.03; Ebeam 34kGy 1.04±0.018; Gamma 34kGy 1.0±0.028; 25kGy: 1.4±2,0; 34kGy: 1.1±1.1). The most important result of this study was that ebeam irradiation showed significantly less impairment of the biomechanical properties than gamma irradiation. Considering the results of this study and the improved control of irradiation application with electronic beam, this technique might be a promising alternative in soft-tissue sterilization. Springer Science+Business Media Dordrecht, 2014 Allograft nationallicence ACL reconstruction nationallicence Sterilization nationallicence Ebeam nationallicence Gamma irradiation nationallicence Hoburg A. Department for Orthopaedic Surgery and Traumatology, Center for Muskuloskeletal Surgery, Sports Medicine and Arthroscopy Service, Charité University Medicine Berlin, Charitéplatz 1, 10117, Berlin, Germany aut Keshlaf S. Institute for Transfusion Medicine (Tissue Bank), Charité University Medicine Berlin, Berlin, Germany aut Schmidt T. Department for Orthopaedic Surgery and Traumatology, Center for Muskuloskeletal Surgery, Sports Medicine and Arthroscopy Service, Charité University Medicine Berlin, Charitéplatz 1, 10117, Berlin, Germany aut Smith M. German Institute for Cell and Tissue Replacement (DIZG), Berlin, Germany aut Gohs U. Leibniz-Institute of Polymer Research Dresden, Hohe Straße 6, 01069, Dresden, Germany aut Perka C. Department for Orthopaedic Surgery and Traumatology, Center for Muskuloskeletal Surgery, Sports Medicine and Arthroscopy Service, Charité University Medicine Berlin, Charitéplatz 1, 10117, Berlin, Germany aut Pruss A. Institute for Transfusion Medicine (Tissue Bank), Charité University Medicine Berlin, Berlin, Germany aut Scheffler S. Chirurgisch Orthopaedischer PraxisVerbund (COPV), Breitenbachplatz 8, 14195, Berlin, Germany aut Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 219-226 1389-9333 16:2<219 2015 16 10561 https://doi.org/10.1007/s10561-014-9461-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9461-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hoburg A. Department for Orthopaedic Surgery and Traumatology, Center for Muskuloskeletal Surgery, Sports Medicine and Arthroscopy Service, Charité University Medicine Berlin, Charitéplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Keshlaf S. Institute for Transfusion Medicine (Tissue Bank), Charité University Medicine Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Schmidt T. Department for Orthopaedic Surgery and Traumatology, Center for Muskuloskeletal Surgery, Sports Medicine and Arthroscopy Service, Charité University Medicine Berlin, Charitéplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Smith M. German Institute for Cell and Tissue Replacement (DIZG), Berlin, Germany aut NATIONALLICENCE 700 1- Gohs U. Leibniz-Institute of Polymer Research Dresden, Hohe Straße 6, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Perka C. Department for Orthopaedic Surgery and Traumatology, Center for Muskuloskeletal Surgery, Sports Medicine and Arthroscopy Service, Charité University Medicine Berlin, Charitéplatz 1, 10117, Berlin, Germany aut NATIONALLICENCE 700 1- Pruss A. Institute for Transfusion Medicine (Tissue Bank), Charité University Medicine Berlin, Berlin, Germany aut NATIONALLICENCE 700 1- Scheffler S. Chirurgisch Orthopaedischer PraxisVerbund (COPV), Breitenbachplatz 8, 14195, Berlin, Germany aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 219-226 1389-9333 16:2<219 2015 16 10561