caa a22 4500 60615499X CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10561-014-9471-8 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9471-8 Isolation of human dermis derived mesenchymal stem cells using explants culture method: expansion and phenotypical characterization [Elektronische Daten] [Jeong-Ran Park, Eunjeong Kim, Jungwon Yang, Hanbyeol Lee, Seok-Ho Hong, Heung-Myong Woo, Sung-Min Park, Sunghun Na, Se-Ran Yang] Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigated to determine the potential of stem cell within human skin dermis. We isolated cells from human dermis, termed here as human dermis-derived mesenchymal stem cells (hDMSCs) which is able to be isolated by using explants culture method. Our method has an advantage over the enzymatic method as it is easier, less expensive and less cell damage. hDMSCs were maintained in basal culture media and proliferation potential was measured. hDMSCs were highly proliferative and successfully expanded with no additional growth factor. In addition, hDMSCs revealed normal karyotype and expressed high levels of CD90, CD73 and CD105 while did not express the surface markers for CD34, CD45 and HLA-DR. Also, we confirmed that hDMSCs possess the capacity to differentiate into multiple lineage including adipocyte, osteocyte, chondrocyte and precursor of hepatocyte lineage. Considering these results, we suggest that hDMSCs might be a valuable source of stem cells and could potentially be a useful source of clinical application. Springer Science+Business Media Dordrecht, 2014 Human dermis nationallicence Mesenchymal stem cells nationallicence Explants culture method nationallicence Differentiation potential nationallicence Park Jeong-Ran Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Kim Eunjeong Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Yang Jungwon Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Lee Hanbyeol Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Hong Seok-Ho Department of Pulmonary Medicine, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Woo Heung-Myong College of Veterinary Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Park Sung-Min Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Na Sunghun Department of Obstetrics and Gynecology, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Yang Se-Ran Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 209-218 1389-9333 16:2<209 2015 16 10561 https://doi.org/10.1007/s10561-014-9471-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9471-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Jeong-Ran Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Eunjeong Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Yang Jungwon Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Lee Hanbyeol Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Hong Seok-Ho Department of Pulmonary Medicine, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Woo Heung-Myong College of Veterinary Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Park Sung-Min Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Na Sunghun Department of Obstetrics and Gynecology, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Yang Se-Ran Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, 200-701, Chuncheon, Republic of Korea aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 209-218 1389-9333 16:2<209 2015 16 10561