caa a22 4500 606155023 CHVBK 20210128100601.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10561-014-9462-9 doi (NATIONALLICENCE)springer-10.1007/s10561-014-9462-9 Induced pluripotent stem cells and their implication for regenerative medicine [Elektronische Daten] [Maria Csobonyeiova, Stefan Polak, Jan Koller, Lubos Danisovic] In 2006 Yamanaka's group showed that stem cells with properties similar to embryonic stem cells could be generated from mouse fibroblasts by introducing four genes. These cells were termed induced pluripotent stem cells (iPSCs). Because iPSCs avoid many of ethical concerns associated with the use of embryonic material, they have great potential in cell-based regenerative medicine. They are suitable also for other various purposes, including disease modelling, personalized cell therapy, drug or toxicity screening and basic research. Moreover, in the future, there might become possible to generate organs for human transplantation. Despite these progresses, several studies have raised the concern for genetic and epigenetic abnormalities of iPSCs that could contribute to immunogenicity of some cells differentiated from iPSCs. Recent methodological improvements are increasing the ease and efficacy of reprogramming, and reducing the genomic modification. However, to minimize or eliminate genetic alternations in the derived iPSC line creation, factor-free human iPSCs are necessary. In this review we discuss recent possibilities of using iPSCs for clinical applications and new advances in field of their reprogramming methods. The main goal of present article was to review the current knowledge about iPSCs and to discuss their potential for regenerative medicine. Springer Science+Business Media Dordrecht, 2014 Induced pluripotent stem cells nationallicence Regenerative medicine nationallicence Gene therapy nationallicence Reprogramming nationallicence Csobonyeiova Maria Institute of Histology and Embryology, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia aut Polak Stefan Institute of Histology and Embryology, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia aut Koller Jan Department of Burns and Reconstructive Surgery, Faculty of Medicine, University Hospital, Comenius University, Pazitkova 4, 821 01, Bratislava, Slovakia aut Danisovic Lubos Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia aut Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 171-180 1389-9333 16:2<171 2015 16 10561 https://doi.org/10.1007/s10561-014-9462-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10561-014-9462-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Csobonyeiova Maria Institute of Histology and Embryology, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Polak Stefan Institute of Histology and Embryology, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Koller Jan Department of Burns and Reconstructive Surgery, Faculty of Medicine, University Hospital, Comenius University, Pazitkova 4, 821 01, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Danisovic Lubos Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia aut NATIONALLICENCE 773 0- Cell and Tissue Banking Springer Netherlands 16/2(2015-06-01), 171-180 1389-9333 16:2<171 2015 16 10561