caa a22 4500 606155198 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10096-014-2203-y doi (NATIONALLICENCE)springer-10.1007/s10096-014-2203-y Highly immunoreactive antibodies against the rHup-F2 fragment (aa 63-161) of the iron-regulated HupB protein of Mycobacterium tuberculosis and its potential for the serodiagnosis of extrapulmonary and recurrent tuberculosis [Elektronische Daten] [N. Sritharan, M. Choudhury, S. Sivakolundu, R. Chaurasia, N. Chouhan, P. Rao, M. Sritharan] HupB is an iron-regulated protein in Mycobacterium tuberculosis that functions as a positive regulator of mycobactin biosynthesis. It is essential for the growth and survival of the pathogen inside macrophages. Previously, using the full-length rHupB of M. tuberculosis, we demonstrated high levels of anti-HupB antibodies in the serum of pulmonary tuberculosis (TB) and, interestingly, extrapulmonary TB patients with negligible levels in household contacts and healthy controls. Here, we used three antigenic fragments of HupB, namely the recombinant HupB-F1 (aa 1-71), HupB-F2 (aa 63-161) and HupB-F3 (aa 164-214), as antigens in enzyme-linked immunosorbent assay (ELISA) to screen serum from TB patients. HupB-F2 showed enhanced immunoreactivity with serum from patients with pulmonary TB (three groups consisting of new cases, defaulters and recurrent cases) and extrapulmonary TB, with negligible levels in normal healthy controls. The negative correlation of the anti-(HupB-F2) antibodies with serum iron was maximal, with a Pearson's correlation coefficient value of −0.415. The study, in addition to strengthening the diagnostic potential of HupB, reflected the superior performance of HupB-F2 as an antigen in screening pulmonary and extrapulmonary TB. Springer-Verlag Berlin Heidelberg, 2014 Sritharan N. NRI Academy of Sciences, Guntur, Andhra Pradesh, India aut Choudhury M. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut Sivakolundu S. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut Chaurasia R. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut Chouhan N. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut Rao P. NRI Academy of Sciences, Guntur, Andhra Pradesh, India aut Sritharan M. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 33-40 0934-9723 34:1<33 2015 34 10096 https://doi.org/10.1007/s10096-014-2203-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2203-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sritharan N. NRI Academy of Sciences, Guntur, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Choudhury M. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut NATIONALLICENCE 700 1- Sivakolundu S. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut NATIONALLICENCE 700 1- Chaurasia R. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut NATIONALLICENCE 700 1- Chouhan N. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut NATIONALLICENCE 700 1- Rao P. NRI Academy of Sciences, Guntur, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Sritharan M. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Prof. CR Rao Road, 500 046, Gachibowli, Hyderabad, India aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 33-40 0934-9723 34:1<33 2015 34 10096