caa a22 4500 606155260 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10096-014-2219-3 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2219-3 Functional synergy of α-helical antimicrobial peptides and traditional antibiotics against Gram-negative and Gram-positive bacteria in vitro and in vivo [Elektronische Daten] [Q. Feng, Y. Huang, M. Chen, G. Li, Y. Chen] In this study, the antimicrobial activities based on the synergistic effects of traditional antibiotics (imipenem, cefepime, levofloxacin hydrochloride and vancomycin) and antimicrobial peptides (AMPs; PL-5, PL-31, PL-32, PL-18, PL-29 and PL-26), alone or in combination, against three Gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae and Staphylococcus epidermidis) and three Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae) were investigated. In addition, the antimicrobial activity that was based on the synergistic effects of levofloxacin hydrochloride and PL-5 against Staphylococcus aureus in vivo was explored in a mouse infection model. Traditional antibiotics and AMPs showed significant synergistic effects on the antibacterial activities against the different Gram-positive and Gram-negative bacteria in vitro. A strong synergistic effect in the PL-5 and levofloxacin hydrochloride combination against Staphylococcus aureus was observed in the mouse infection model in vivo. The mechanism of synergistic action was due to the different targets of AMPs and traditional antibiotics. The combination of AMPs and traditional antibiotics can dramatically enhance antimicrobial activity and may help prevent or delay the emergence of antibiotic resistance. Thus, this combination therapy could be a promising approach to treat bacterial infections, particularly mixed infections and multi-antibiotic-resistant infections, in the clinics. Springer-Verlag Berlin Heidelberg, 2014 Feng Q. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut Huang Y. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut Chen M. Changchun ProteLight Pharmaceutical & Biotechnology Co., Ltd., Changchun, China aut Li G. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut Chen Y. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 197-204 0934-9723 34:1<197 2015 34 10096 https://doi.org/10.1007/s10096-014-2219-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2219-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Q. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Huang Y. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Chen M. Changchun ProteLight Pharmaceutical & Biotechnology Co., Ltd., Changchun, China aut NATIONALLICENCE 700 1- Li G. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Chen Y. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin St., 130012, Changchun, Jilin, China aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 197-204 0934-9723 34:1<197 2015 34 10096