caa a22 4500 606155287 CHVBK 20210128100602.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10096-014-2192-x doi (NATIONALLICENCE)springer-10.1007/s10096-014-2192-x New options of antibiotic combination therapy for multidrug-resistant Pseudomonas aeruginosa [Elektronische Daten] [I. Nakamura, T. Yamaguchi, A. Tsukimori, A. Sato, S. Fukushima, T. Matsumoto] Several antibiotic combinations have demonstrated increased activity against multidrug-resistant Pseudomonas aeruginosa (MDRP) in vitro compared with a single antibiotic. The aim of this study was to investigate the activity against MDRP of some aminoglycosides in combination with monobactam, piperacillin (PIPC), and carbapenem. Clinical isolates of MDRP were collected between November 2010 and October 2012 from patients in Tokyo Medical University Hospital, Tokyo (1,015 beds). Our new method was designed to evaluate three concentrations around the breakpoint of each drug using the Checkerboard method. The aminoglycosides tested were amikacin (AMK), tobramycin (TOB), and arbekacin (ABK). Ciprofloxacin, PIPC, and biapenem (BIPM), which have been reported to demonstrate combination effects, were also tested. Sixty-six MDRP strains were identified from the 2,417 P. aeruginosa strains. Of the 66, 27 tested positive for metallo-β-lactamase (MBL). Aztreonam (AZT) with AMK or ABK was the most effective against MDRP. PIPC with AMK or ABK were somewhat effective. AZT with AMK or ABK were more effective against MBL-positive strains than MBL-negative strains. However, PIPC with AMK or ABK were more effective against MBL-negative strains than MBL-positive strains. Combination activities showed differences between MBL-positive and MBL-negative strains. Springer-Verlag Berlin Heidelberg, 2014 Nakamura I. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut Yamaguchi T. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut Tsukimori A. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut Sato A. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut Fukushima S. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut Matsumoto T. Department of Microbiology, Tokyo Medical University, Tokyo, Japan aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 83-87 0934-9723 34:1<83 2015 34 10096 https://doi.org/10.1007/s10096-014-2192-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2192-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura I. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamaguchi T. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsukimori A. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut NATIONALLICENCE 700 1- Sato A. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut NATIONALLICENCE 700 1- Fukushima S. Department of Infection Control and Prevention, Tokyo Medical University Hospital, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsumoto T. Department of Microbiology, Tokyo Medical University, Tokyo, Japan aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 83-87 0934-9723 34:1<83 2015 34 10096